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- PURPOSEpAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.
- DEPOSITING LAB
BACKBONE
- Vector backbonepAAV
Backbone size w/o insert (bp)2905
- Vector typeMammalian Expression, AAV, CRISPR
GROWTH IN BACTERIA
- Bacterial Resistance(s)Ampicillin
- Growth Temperature37°C
- Growth Strain(s)Stbl3
- Copy numberHigh Copy
SEQUENCE INFORMATION
- Depositor Sequences
- Addgene Sequences
GENE/INSERT
- Gene/Insert nameSpCas9
- Alt nameS. pyogenes Cas9 nuclease
- Alt nameCas9
- SpeciesSynthetic
- Insert Size (bp)4200
PromoterpMecp2
Tag / Fusion Protein
HA (N terminal on insert)
CLONING INFORMATION
Cloning methodRestriction Enzyme
5′ cloning siteAgeI (not destroyed)
3′ cloning siteEcoRI (not destroyed)
5′ sequencing primerAATGGGGTCCGCCTCTTTTCC
3′ sequencing primercttagctggcctccacctttctcttc
(Common Sequencing Primers)
RESOURCE INFORMATION
How to cite this plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:
PX551 was a gift from Feng Zhang (Addgene plasmid # 60957)For your References section:
In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9. Swiech L, Heidenreich M, Banerjee A, Habib N, Li Y, Trombetta J, Sur M, Zhang F. Nat Biotechnol. 2014 Oct 19. doi: 10.1038/nbt.3055.10.1038/nbt.3055 PubMed 25326897
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