雌二醇誘導的植物熒光表達載體/雙分子熒光互補載體質粒pABindGFP, pABindmCherry,pABindFRET.

Inducible transgene expression rescues the corresponding mutants. A, Speculative model for interactions of CLV3 with receptor complexes. CLV3 peptide is proposed to bind to two separate receptor systems, consisting of CLV1 and CLV2 together with CRN. CLV2 homodimers could interact with CRN via their transmembrane domains. Receptor activity restricts stem cell fate, and the separate receptor complexes may interact through their kinase domains. B, T-DNA for inducible expression of translational fusions with the FPs GFP, mCherry, or both. G10-90, Constitutive promoter; XVE, chimeric transcription factor that activates transcription from the lexA-46 35S promoter upon estradiol induction. FPs are GFP in pABindGFP, mCherry in pABindmCherry, and GFP-mCherry in pABindFRET. C, Schematic representation of CLV1-FP, CLV2-FP, and CRN-FP. D to F, Examples of partial phenotypic restoration in clv1-11, clv2-1, and crn-1 mutants upon induced expression of the corresponding FP fusion protein. Left panels show whole plants; top insets show higher magnification of the primary shoot; bottom insets show mutant siliques with four carpels and rescued siliques (asterisks) with two carpels. D, clv1-11 carrying the iCLV1-GFP transgene. InducingiCLV1-GFP expression led to the formation of a single silique with two carpels, whereas older and younger siliques form four carpels. E, clv2-1carrying iCLV2-GFP. iCLV2-GFP induction restored carpel number to two in four siliques. F, crn-1 carrying iCRN-GFP. iCRN-GFP induction led to the formation of three siliques with only two carpels; all older and younger siliques consisted of four carpels. Bars = 1 cm.

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