LOCUS       pRetroX-IRES-DsRedExpress   5934 bp    DNA   circular            10-NOV-2009
COMMENT     Created by Clontech Laboratories Inc.
COMMENT     Cat. No. 632521
COMMENT     This file is created by Vector NTI
            http://www.biovector.net/
COMMENT     VNTAUTHORNAME|biovector.net|
FEATURES             Location/Qualifiers
     LTR             1..592
                     /label=5'\MMLV\LTR
     misc_feature    662..1012
                     /label=Packaging\signal
     intron          1075..1308
                     /label=Intron\containing\splice\acceptor\site
     LTR             2699..3290
                     /label=3'\MMLV\LTR
     rep_origin      4143..4262
                     /label=pUC\origin
     CDS             complement(4470..5327)
                     /label=Amp(R)
     misc_feature    1392..1966
                     /label=IRES
     misc_feature    1967..2644
                     /label=DsRedExpress
     misc_feature    1361..1390
                     /label=MCS
     misc_feature    650..655
                     /label=Splice\donor\site
BASE COUNT     1402 a      1571 c      1557 g      1404 t 
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat 
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca gatggaacag 
      121 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa 
      181 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc 
      241 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag 
      301 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg 
      361 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgggctcaat 
      421 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gattgactga gtcgcccggg 
      481 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggcct cgctgttcct 
      541 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct 
      601 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg 
      661 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct 
      721 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga 
      781 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg 
      841 tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca ccccccttag 
      901 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg 
      961 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg 
     1021 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatacg cgtgtcgagc 
     1081 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac 
     1141 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa 
     1201 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt 
     1261 ttttttcttc catttcagcc gtcgtgaaaa ctacccctaa aagccaaaaa aatcgaattc 
     1321 ccaaacttaa gcttggtacc gagctcggat ctcacgtggg cccgcggccg cagatctatc 
     1381 gatggatccg tgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga 
     1441 ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa 
     1501 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc 
     1561 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc 
     1621 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg 
     1681 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca 
     1741 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tcacctcaag 
     1801 cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct 
     1861 ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaacg tctaggcccc 
     1921 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg cctcctccga 
     1981 ggacgtcatc aaggagttca tgcgcttcaa ggtgcgcatg gagggctccg tgaacggcca 
     2041 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa 
     2101 gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt 
     2161 ccagtacggc tccaaggtgt acgtgaagca ccccgccgac atccccgact acaagaagct 
     2221 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt 
     2281 gaccgtgacc caggactcct ccctgcagga cggctccttc atctacaagg tgaagttcat 
     2341 cggcgtgaac ttcccctccg acggccccgt aatgcagaag aagactatgg gctgggaggc 
     2401 ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc acaaggccct 
     2461 gaagctgaag gacggcggcc actacctggt ggagttcaag tctatctata tggccaagaa 
     2521 gcccgtgcag ctgcccggct actactacgt ggactccaag ctggacatca cctcccacaa 
     2581 cgaggactac accatcgtgg agcagtacga gcgcgccgag ggccgccacc acctgttcct 
     2641 gtagaatgct cgaggataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg 
     2701 aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg 
     2761 aaaaatacat aactgagaat agagaagttc agatcaaggt caggaacaga tggaacagct 
     2821 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga 
     2881 acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc 
     2941 ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag 
     3001 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa 
     3061 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa 
     3121 aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta 
     3181 cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg 
     3241 ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt gaattcgtaa 
     3301 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 
     3361 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 
     3421 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 
     3481 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 
     3541 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 
     3601 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 
     3661 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 
     3721 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 
     3781 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 
     3841 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 
     3901 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 
     3961 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 
     4021 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 
     4081 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 
     4141 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 
     4201 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 
     4261 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 
     4321 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 
     4381 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 
     4441 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 
     4501 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 
     4561 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 
     4621 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 
     4681 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 
     4741 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 
     4801 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 
     4861 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 
     4921 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 
     4981 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 
     5041 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 
     5101 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 
     5161 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 
     5221 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 
     5281 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 
     5341 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 
     5401 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 
     5461 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc 
     5521 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag 
     5581 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca 
     5641 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg 
     5701 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc 
     5761 aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct 
     5821 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg 
     5881 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct tatg 
//