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pEGFPN1載體基本信息
| 出品公司: | Clontech |
|---|---|
| 別名: | pEGFP-N1, pEGFP N1 |
| 質粒類型: | 哺乳動物細胞表達載體 |
| 啟動子: | CMV |
| 表達水平: | 高 |
| 克隆方法: | 多克隆位點，限制性內切酶 |
| 載體大小: | 4733bp |
| 5' 測序引物: | CMV-F |
| 5' 測序引物序列: | 5'-CGCAAATGGGCGGTAGGCGTG-3' |
| 3' 測序引物: | EGFP-N |
| 3' 測序引物序列: | 5'-CGTCGCCGTCCAGCTCGACCAG-3' |
| 載體標簽: | C-EGFP |
| 載體抗性: | 卡那 |
| 篩選標記: | 新霉素 (G418) |
| 備注: | Clontech公司已經終止了該載體的生產和銷售. |
| 產品目錄號: | 6085-1 |
| 穩定性: | 穩表達 |
| 組成型: | 組成型 |
| 病毒/非病毒: | 非病毒 |
pEGFPN1載體質粒圖譜和多克隆位點信息
pEGFPN1載體簡介
pEGFP-N1 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm;emission maximum = 507 nm.) pEGFP-N1 encodes the GFPmut1 variant (4) which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr. The coding sequence of the EGFP gene contains more than 190 silent base changes which correspond to human codon-usage preferences (5). Sequences flanking EGFP have been converted to a Kozak consensus translation initiation site (6) to further increase the translation efficiency in eukaryotic cells. The MCS in pEGFP-N1 is between the immediate early promoter of CMV (PCMV IE) and the EGFP coding sequences. Genes cloned into the MCS will be expressed as fusions to the N-terminus of EGFP if they are in the same reading frame as EGFP and there are no intervening stop codons. SV40polyadenylation signals downstream of the EGFP gene direct proper processing of the 3' end of the EGFP mRNA. The vector backbone also contains an SV40 origin for replication in mammalian cells expressing the SV40 T antigen. A neomycin-resistance cassette (Neor), consisting of the SV40 early promoter, the neomycin/kanamycin resistance gene of Tn5, and polyadenylation signals from the Herpes simplex virus thymidine kinase (HSV TK) gene, allows stably transfected eukaryotic cells tobe selected using G418. A bacterial promoter upstream of this cassette expresses kanamycin resistance in E. coli. The pEGFP-N1 backbone also provides a pUC origin of replication for propagation in E. coli and an f1 origin for single-stranded DNA production.
Use:
Fusions to the N terminus of EGFP retain the fluorescent properties of the native protein allowing the localization of the fusion protein in vivo . The target gene should be cloned into pEGFP-N1 so that it is in frame with the EGFP coding sequences, with no intervening in-frame stop codons. The inserted gene should include the initiating ATG codon. The recombinant EGFP vector can be transfected into mammalian cells using any standard transfection method. If required, stable transformants can be selected using G418 (7). pEGFP-N1 can also be used simply to express EGFP in a cell line of interest (e.g., as a transfection marker).
pEGFPN1載體序列
LOCUS pEGFP-N1 4733 bp DNA Circular SYN
DEFINITION pEGFP-N1
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biovector.net/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biovector.net|
FEATURES Location/Qualifiers
source 1..4733
/organism="pEGFP-N1"
/mol_type="other DNA"
promoter 10..562
/label="CMV_immearly_promoter"
misc_feature 65..352
/label="CAG_enhancer"
misc_feature 519..539
/label="CMV_fwd_primer"
promoter 520..589
/label="CMV_promoter"
CDS 679..1398
/label="ORF frame 1"
gene 682..1395
/label="EGFP"
/gene="EGFP"
misc_feature complement(724..745)
/label="EGFP_N_primer"
misc_feature 862..891
/label="Y66 (EGFP)"
misc_feature 1332..1353
/label="EGFP_C_primer"
misc_feature 1612..1631
/label="EBV_rev_primer"
rep_origin complement(1781..2087)
/label="f1_origin"
promoter 2166..2194
/label="AmpR_promoter"
misc_feature complement(2260..2280)
/label="pBABE_3_primer"
misc_feature complement(2266..2481)
/label="SV40_enhancer"
promoter 2278..2546
/label="SV40_promoter"
rep_origin 2445..2522
/label="SV40_origin"
misc_feature 2507..2526
/label="SV40pro_F_primer"
CDS 2629..3423
/label="ORF frame 1"
gene 2632..3420
/label="NeoR/KanR"
/gene="NeoR/KanR"
CDS complement(2938..3474)
/label="ORF frame 3"
terminator 3598..3867
/label="TK_PA_terminator"
rep_origin 4015..4634
/label="pBR322_origin"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
661 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
721 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
781 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
841 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
901 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
961 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
1021 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
1081 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
1141 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
1201 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
1261 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
1321 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
1381 gacgagctgt acaagtaaag cggccgcgac tctagatcat aatcagccat accacatttg
1441 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
1501 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
1561 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
1621 ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc
1681 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
1741 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
1801 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
1861 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
1921 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
1981 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
2041 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
2101 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
2161 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
2221 tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
2281 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
2341 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
2401 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
2461 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
2521 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
2581 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
2641 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
2701 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
2761 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
2821 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
2881 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
2941 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
3001 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
3061 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
3121 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc
3181 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
3241 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
3301 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
3361 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
3421 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
3481 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
3541 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg
3601 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
3661 taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt
3721 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
3781 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
3841 acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt tagattgatt
3901 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
3961 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
4021 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
4081 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
4141 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
4201 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
4261 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
4321 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
4381 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
4441 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
4501 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
4561 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
4621 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
4681 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg cat您正在向 biovector.net 發送關于產品 pEGFP-N1質粒圖譜序列抗性價格報價Biovector NTCC Inc.現貨 的詢問
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