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pBI CMV1載體基本信息
| 出品公司: | Clontech |
|---|---|
| 載體名稱: | pBI-CMV1, pBI CMV1 |
| 質粒類型: | 哺乳動物細胞雙向表達載體 |
| 啟動子: | CMV |
| 表達水平: | 高 |
| 克隆方法: | 多克隆位點，限制性內切酶 |
| 載體大小: | 3114bp |
| 5' 測序引物: | CMV-F |
| 5' 測序引物序列: | 5'-CGCAAATGGGCGGTAGGCGTG-3' |
| 載體標簽: | 無 |
| 載體抗性: | Ampicillin |
| 備注: | 該載體是一個哺乳動物細胞雙向表達載體，可以同時組成型的表達兩個目的基因。 |
| 產品目錄號: | 631630 |
| 穩定性: | 穩表達 Stable |
| 組成型: | 組成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
pBI CMV1載體質粒圖譜和多克隆位點信息
| Feature Name | Start | End | |
|---|---|---|---|
| Enhancer | 64 | 473 | |
| P minCMV1 | 474 | 599 | |
| Multiple cloning site 1 | 602 | 663 | |
| SV40 polyA signals | 675 | 862 | |
| ColE1 origin of replication | 1038 | 1637 | |
| AmpR (ampicillin resistance gene) | 2659 | 1799 | |
| SV40 polyA signals | 2982 | 2795 | |
| Multiple cloning site 2 | 2986 | 3040 | |
| P minCMV2 | 3046 | 3114 |
pBI CMV1載體簡介
Description:
pBI-CMV1 is a mammalian bidirectional expression vector that allows the constitutive expression of two proteins of interest. Protein expression is driven by one of two constitutively active, minimal human cytomegalovirus promoters, PminCMV1 and PminCMV2, located just upstream of two independent multiple cloning sites (MCS 1 and MCS 2, respectively). To allow propagation and selection in E. coli, the vector also contains a ColE1 origin of replication and an ampicillin resistance gene (Ampr).
Use:
pBI-CMV1 is designed to simultaneously and constitutively express two genes of interest. Each gene must be cloned into a different MCS, and contain both an initiation codon and a stop codon. The pBI-CMV1 vector can be transfected into mammalian cells using any standard transfection method.
pBI CMV1載體序列
LOCUS pBI-CMV1 3114 bp ds-DNA circular SYN
DEFINITION Mammalian bidirectional promoter vector for constitutively
expressing two genes at similar levels.
ACCESSION .
VERSION .
KEYWORDS pBI-CMV1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
COMMENT This file is created by Vector NTI
http://www.biovector.net/
COMMENT ORIGDB|GenBank|
COMMENT VNTAUTHORNAME|biovector.net|
COMMENT The gene inserted into each MCS should contain start and stop
codons.
FEATURES Location/Qualifiers
source 1..3114
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 67..370
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 371..574
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 602..663
/label="MCS 1"
/note="MCS 1"
/note="multiple cloning site 1"
polyA_signal 777..858
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1040..1628)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1799..2659)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2660..2764)
/gene="bla"
/note="AmpR promoter"
polyA_signal 2797..2878
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
misc_feature 2986..3040
/label="MCS 2"
/note="MCS 2"
/note="multiple cloning site 2"
promoter complement(3069..3106)
/note="minimal CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
ORIGIN
1 ctcgagtagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
601 gggatcctct agtcagctga cgcgtgctag cgcggccgca tcgataagct tgtcgacgat
661 atctccagag gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
721 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
781 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
841 aagcattttt ttcactgccc cgagcttcct cgctcactga ctcgctgcgc tcggtcgttc
901 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
961 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
1021 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
1081 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
1141 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
1201 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
1261 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
1321 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
1381 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
1441 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
1501 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
1561 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
1621 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
1681 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
1741 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
1801 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
1861 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
1921 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
1981 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
2041 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
2101 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
2161 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
2221 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
2281 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
2341 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
2401 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
2461 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
2521 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
2581 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
2641 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
2701 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
2761 cgcgcacatt tccccgaaaa gtgccacctg acgtcggcag tgaaaaaaat gctttatttg
2821 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
2881 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
2941 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag actgcagcct
3001 caggagatct gggcccccgc ggcatatgac cggtgaattc tccaggcgat ctgacggttc
3061 actaaacgag ctctgcttat ataggcctcc caccgtacac gccacctcga cata
DEFINITION Mammalian bidirectional promoter vector for constitutively
expressing two genes at similar levels.
ACCESSION .
VERSION .
KEYWORDS pBI-CMV1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
COMMENT This file is created by Vector NTI
http://www.biovector.net/
COMMENT ORIGDB|GenBank|
COMMENT VNTAUTHORNAME|biovector.net|
COMMENT The gene inserted into each MCS should contain start and stop
codons.
FEATURES Location/Qualifiers
source 1..3114
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 67..370
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 371..574
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 602..663
/label="MCS 1"
/note="MCS 1"
/note="multiple cloning site 1"
polyA_signal 777..858
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1040..1628)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1799..2659)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2660..2764)
/gene="bla"
/note="AmpR promoter"
polyA_signal 2797..2878
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
misc_feature 2986..3040
/label="MCS 2"
/note="MCS 2"
/note="multiple cloning site 2"
promoter complement(3069..3106)
/note="minimal CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
ORIGIN
1 ctcgagtagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
601 gggatcctct agtcagctga cgcgtgctag cgcggccgca tcgataagct tgtcgacgat
661 atctccagag gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
721 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
781 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
841 aagcattttt ttcactgccc cgagcttcct cgctcactga ctcgctgcgc tcggtcgttc
901 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
961 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
1021 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
1081 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
1141 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
1201 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
1261 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
1321 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
1381 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
1441 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
1501 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
1561 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
1621 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
1681 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
1741 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
1801 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
1861 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
1921 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
1981 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
2041 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
2101 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
2161 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
2221 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
2281 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
2341 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
2401 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
2461 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
2521 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
2581 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
2641 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
2701 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
2761 cgcgcacatt tccccgaaaa gtgccacctg acgtcggcag tgaaaaaaat gctttatttg
2821 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
2881 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
2941 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag actgcagcct
3001 caggagatct gggcccccgc ggcatatgac cggtgaattc tccaggcgat ctgacggttc
3061 actaaacgag ctctgcttat ataggcctcc caccgtacac gccacctcga cata
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