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Full plasmid sequence is available only if provided by the depositing laboratory. A cDNA encoding the TAP tag was PCR-cloned from the plasmid pBSactTAP with the following primers: left primer 5 -GGACAATG ATCAGGCACCATGGCAGGCCTTGCGCAACACG-3 and right primer 5 -CCGGAATTCCCGGCGGCCGCGGGATCCATTCGCTCGAGGGAC CACCACCACCACCAAGTGCCCCGGAGGATGAG-3 . The PCR product was then cut with BclI/EcoRI and inserted into pMSCV-puro (Clontech) cut with BglII/EcoRI. See authors map for more information (Note that the NotI site is not precisely underlined in the authors map). These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications. For your Materials & Methods section: For your References section:pMSCV-TAP
(BioVector-012570)
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