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pCLE
(BioVector-017703)-
PURPOSE(Empty Backbone)
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DEPOSITING LAB
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PUBLICATION
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PRICE$65 (USD)
Add to CartAvailable to Academic and Nonprofits OnlyBACKBONE
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Vector backbonepCLE
- Backbone size (bp)7600
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Vector typeMammalian Expression, Retroviral
GROWTH IN BACTERIA
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
SEQUENCE INFORMATION
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Depositor SequencesNone.
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Addgene Sequences
Full plasmid sequence is available only if provided by the depositing laboratory.
GENE/INSERT
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Gene/Insert nameNone
CLONING INFORMATION
- Cloning methodRestriction Enzyme
- 5′ cloning siteBglII (not destroyed)
- 3′ cloning siteBglII (not destroyed)
- 5′ sequencing primerna (Common Sequencing Primers)
RESOURCE INFORMATION
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Terms and Licenses
DEPOSITOR COMMENTS
pCLE includes a modified 5 LTR (CMV/MLV) to obtain higher titers, as well as the Xenopus EF1alpha enhancer/promoter to improve vector expression in the brain. In addition, the vector is dicistronic and utilizes an IRES to allow translation of the reporter gene, human placental alkaline phosphatase (PLAP).
How to cite this plasmidThese plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCLE was a gift from Nicholas Gaiano (Addgene plasmid # 17703) -
For your References section:
Radial glial identity is promoted by Notch1 signaling in the murine forebrain. Gaiano N, Nye JS, Fishell G. Neuron. 2000 May . 26(2):395-404. 10.1016/S0896-6273(00)81172-1 PubMed 10839358
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