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Plasmid: pMXs
Analyze:
Sequence
Plasmid Type:
Mammalian Expression, Retroviral
Cloning Method:
Unknown
5 Sequencing 1 Primer:
GAC GGC ATC GCA GCT TGG ATA CAC (you can also use pBMN5)
Bacterial Resistance:
Ampicillin
Notes:
Described in Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H. Unlike most other retrovirus vectors such as LXSN and MSCV, the pMX vector harbors the splicing donor and acceptor sites and produces two types of transcripts, the full-length genome RNA and the subgenomic RNA. Therefore, in addition to the correct proteins produced from the subgenomic RNA, Gag-fusion proteins could be expressed from the full-length genome RNA if the reading frame of gag and that of the inserted gene match and there is no stop codon between them. To generate improved vectors free of the Gag and the Gag-fusion proteins, we disrupted the ATG start codon of gag, inserted a stop codon downstream of the CTG start codon of glyco gag, and inserted triple stop codons in three different reading frames just before MCS. The improved vectors are termed pMXs. A variety of pMXs series are depicted in the included map, including pMXs-puro, pMXs-neo, pMXs-IG (IRES-GFP), pMXs-IN (IRES-neo), and pMXs-IP (IRES-puro). pMYs, pMZs, and pMCs are also described in this publication.
Stable:
Unspecified
Constitutive:
Unspecified
Viral/Non-Viral:
Retroviral
| Analyze: | Sequence |
| Plasmid Type: | Mammalian Expression, Retroviral |
| Cloning Method: | Unknown |
| 5 Sequencing 1 Primer: | GAC GGC ATC GCA GCT TGG ATA CAC (you can also use pBMN5) |
| Bacterial Resistance: | Ampicillin |
| Notes: |
Described in Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H. Unlike most other retrovirus vectors such as LXSN and MSCV, the pMX vector harbors the splicing donor and acceptor sites and produces two types of transcripts, the full-length genome RNA and the subgenomic RNA. Therefore, in addition to the correct proteins produced from the subgenomic RNA, Gag-fusion proteins could be expressed from the full-length genome RNA if the reading frame of gag and that of the inserted gene match and there is no stop codon between them. To generate improved vectors free of the Gag and the Gag-fusion proteins, we disrupted the ATG start codon of gag, inserted a stop codon downstream of the CTG start codon of glyco gag, and inserted triple stop codons in three different reading frames just before MCS. The improved vectors are termed pMXs. A variety of pMXs series are depicted in the included map, including pMXs-puro, pMXs-neo, pMXs-IG (IRES-GFP), pMXs-IN (IRES-neo), and pMXs-IP (IRES-puro). pMYs, pMZs, and pMCs are also described in this publication.
|
| Stable: | Unspecified |
| Constitutive: | Unspecified |
| Viral/Non-Viral: | Retroviral |
Generated Plasmid Map
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