pREG(R1-R4) #BGV059 
(BioVector-048991)

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• PURPOSE

  (Empty Backbone) Retroviral Destination vector containing AttR1-AttR4 site. Use with 4-way LR reaction with cDNA in entry vector, marker in pBEG R2-L3 plasmids and shRNA in pBEG R3-L4 plasmid (module 2,3,and 4)
• DEPOSITING LAB

  David Dankort
• PUBLICATION

  Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi: 10.1371/journal.pone.0076279.
• PRICE

  $65 (USD)

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Available to Academic and Nonprofits Only

BACKBONE

• Vector backbone
  gQxiPuro
  (Search Vector Database)
• Backbone manufacturer
  Dankort lab, unpublished
• Backbone size (bp)7435
• Modifications to backbone
  The R1-R4 Gateway cassette was excised with SacII/HpaI and ligated into gQxiPuro at SacII/EcoRV sites.
• Vector type
  Retroviral
• PromoterCMV

GROWTH IN BACTERIA

• Bacterial Resistance(s)
  Ampicillin and Chloramphenicol
• Growth Temperature
  37°C
• Growth Strain(s)
  ccdB Survival
• Copy number
  Unknown

SEQUENCE INFORMATION

• Depositor Sequences
  Partial (1)
• Addgene Sequences
  Partial (3)

Full plasmid sequence is available only if provided by the depositing laboratory.

CLONING INFORMATION

• Cloning methodGateway Cloning
• 5′ sequencing primerCMV-F
(Common Sequencing Primers)

RESOURCE INFORMATION

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