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- 已注冊
pCEP4 is an episomal mammalian expression vector that uses the cytomegalovirus (CMV) immediate early enhancer/promoter for high level transcription of recombinant genes inserted into the multiple cloning site. The Epstein-Barr Virus replication origin (oriP) and nuclear antigen (encoded by the EBNA-1 gene) is carried by this plasmid to permit extrachromosomal replication in human, primate, and canine cells. pCEP4 also carries the hygromycin B resistance gene for stable selection in transfected cells. pCEP4/CAT is provided as a positive control for the relative level of expression of recombinant proteins in a cell line of interest. It expresses the chloramphenicol acetyl transferase (CAT) protein from the CMV enhancer/promoter. Like its parent vector pCEP4, pCEP4/CAT contains the hygromycin B resistance gene for selection.
載體基本信息
| Invitrogen |
| pCEP4 |
| 哺乳動物細胞表達載體 |
| CMV |
| 高 |
| 多克隆位點，限制性內切酶 |
| 10410 bp |
| CMV-F: 5'-CGCAAATGGGCGGTAGGCGTG-3' |
| EBV-R:5'-GTGGTTTGTCCAAACTCATC-3' |
| / |
| 氨芐 |
| 潮霉素 |
| V044-50 |
| 穩表達 |
| 組成型 |
| 非病毒 |
載體質粒圖譜和多克隆位點信息
載體簡介
pCEP4 is an episomal mammalian expression vector that uses the cytomegalovirus (CMV) immediate early enhancer/promoter for high level transcription of recombinant genes inserted into the multiple cloning site. The Epstein-Barr Virus replication origin (oriP) and nuclear antigen (encoded by the EBNA-1 gene) is carried by this plasmid to permit extrachromosomal replication in human, primate, and canine cells. pCEP4 also carries the hygromycin B resistance gene for stable selection in transfected cells. pCEP4/CAT is provided as a positive control for the relative level of expression of recombinant proteins in a cell line of interest. It expresses the chloramphenicol acetyl transferase (CAT) protein from the CMV enhancer/promoter. Like its parent vector pCEP4, pCEP4/CAT contains the hygromycin B resistance gene for selection.
載體序列
LOCUS pCEP4 10410 bp DNA circular SYN
DEFINITION pCEP4
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..10410
/organism="pCEP4"
/mol_type="other DNA"
misc_feature 112..134
/label="pGEX_3_primer"
terminator 279..410
/label="SV40_PA_terminator"
misc_feature 367..386
/label="EBV_rev_primer"
promoter complement(474..555)
/label="CMV_promoter"
misc_feature complement(474..498)
/label="LNCX_primer"
misc_feature complement(481..500)
/label="pCEP_fwd_primer"
misc_feature complement(524..544)
/label="CMV_fwd_primer"
CDS 1305..2045
/label="ORF frame 3"
terminator complement(1566..1836)
/label="TK_PA_terminator"
CDS complement(1844..2869)
/label="ORF frame 3"
gene complement(1847..2854)
/label="hygroB"
/gene="hygroB"
CDS 2111..3481
/label="ORF frame 2"
promoter 2988..3136
/label="TATA_promoter"
gene 3143..3481
/label="tet (852 - 1190)"
/gene="tet (852 - 1190)"
CDS 3385..3996
/label="ORF frame 1"
gene complement(4430..5290)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(4430..5290)
/label="ORF frame 3"
promoter complement(5332..5360)
/label="AmpR_promoter"
CDS complement(5914..7128)
/label="ORF frame 1"
CDS 5916..7841
/label="ORF frame 3"
CDS 6031..7143
/label="ORF frame 1"
rep_origin 8145..10315
/label="EBV_origin"
ORIGIN
1 tctagagtcg accggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
61 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
121 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagccg gatcataatc
181 agccatacca catttgtaga ggttttactt gctttaaaaa acctccccac ctccccctga
241 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
301 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
361 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cggccttgcc
421 ggcctcgagc ggccgctagc aagcttgcta gcagctggta cccagcttct agagatctga
481 cggttcacta aacgagctct gcttatatag acctcccacc gtacacgcct accgcccatt
541 tgcgtcaacg gggcggggtt attacgacat tttggaaagt cccgttgatt ttggtgccaa
601 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
661 tatccacgcc cattggtgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
721 acgtagatgt actgccaagt aggaaagtcc cgtaaggtca tgtactgggc ataatgccag
781 gcgggccatt taccgtcatt gacgtcaata gggggcggac ttggcatatg atacacttga
841 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
901 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
961 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
1021 ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa
1081 catggcggtc atattggaca tgagccaata taaatgtaca tattatgata tagatacaac
1141 gtatgcaatg gccaatagcc aatattgatt tatgctatat aaccaatgac taatatggct
1201 aattgccaat attgattcaa tgtatagatc ttccatacct accagttctg cgcctgcagc
1261 aatgcaacaa cgttgcccgg atctgcgatg ataagctgtc aaacatgaga attggtcgac
1321 tagcttggca cgccagaaat ccgcgcggtg gtttttgggg gtcgggggtg tttggcagcc
1381 acagacgccc ggtgttcgtg tcgcgccagt acatgcggtc catgcccagg ccatccaaaa
1441 accatgggtc tgtctgctca gtccagtcgt ggaccagacc ccacgcaacg cccaaaataa
1501 taacccccac gaaccataaa ccattcccca tgggggaccc cgtccctaac ccacggggcc
1561 agtggctatg gcagggcctg ccgccccgac gttggctgcg agccctgggc cttcacccga
1621 acttgggggg tggggtgggg aaaaggaaga aacgcgggcg tattggcccc aatggggtct
1681 cggtggggta tcgacagagt gccagccctg ggaccgaacc ccgcgtttat gaacaaacga
1741 cccaacaccc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg
1801 gtattgtctc cttccgtgtt tcagttagcc tcccccatct cccctattcc tttgccctcg
1861 gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc
1921 agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga
1981 cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc
2041 tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg
2101 caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg
2161 gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc
2221 agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg
2281 cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag
2341 cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg
2401 gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc
2461 cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatggcct
2521 ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac
2581 cctgtgcacg gcgggagatg caataggtca ggctctcgct gaattcccca atgtcaagca
2641 cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga
2701 aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga
2761 aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt
2821 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tctcattgcc
2881 cgggatctgc ggcacgctgt tgacgctgtt aagcgggtcg ctgcagggtc gctcggtgtt
2941 cgaggccaca cgcgtcacct taatatgcga agtggacctg ggaccgcgcc gccccgactg
3001 catctgcgtg ttcgaattcg ccaatgacaa gacgctgggc ggggtttgtg tcatcataga
3061 actaaagaca tgcaaatata tttcttccgg ggacaccgcc agcaaacgcg agcaacgggc
3121 cacggggatg aagcagggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc
3181 gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat
3241 gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca
3301 aggatcgctc gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc
3361 gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct
3421 ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg
3481 aatggaagcc ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa
3541 ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc
3601 gccatctcca gcagccgcac gcggcgcagc aaaaggccag gaaccgtaaa aaggccgcgt
3661 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
3721 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
3781 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
3841 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
3901 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
3961 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
4021 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
4081 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
4141 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
4201 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
4261 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
4321 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
4381 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
4441 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
4501 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
4561 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
4621 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
4681 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
4741 ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
4801 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
4861 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
4921 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
4981 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
5041 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
5101 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
5161 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
5221 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
5281 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
5341 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
5401 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
5461 aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt ctcatgtttg acagcttatc
5521 atcgataagc tgatcctcac aggccgcacc cagcttttct tccgttgccc cagtagcatc
5581 tctgtctggt gaccttgaag aggaagagga ggggtcccga gaatccccat ccctaccgtc
5641 cagcaaaaag ggggacgagg aatttgaggc ctggcttgag gctcaggacg caaatcttga
5701 ggatgttcag cgggagtttt ccgggctgcg agtaattggt gatgaggacg aggatggttc
5761 ggaggatggg gaattttcag acctggatct gtctgacagc gaccatgaag gggatgaggg
5821 tgggggggct gttggagggg gcaggagtct gcactccctg tattcactga gcgtcgtcta
5881 ataaagatgt ctattgatct cttttagtgt gaatcatgtc tgacgagggg ccaggtacag
5941 gacctggaaa tggcctagga gagaagggag acacatctgg accagaaggc tccggcggca
6001 gtggacctca aagaagaggg ggtgataacc atggacgagg acggggaaga ggacgaggac
6061 gaggaggcgg aagaccagga gccccgggcg gctcaggatc agggccaaga catagagatg
6121 gtgtccggag accccaaaaa cgtccaagtt gcattggctg caaagggacc cacggtggaa
6181 caggagcagg agcaggagcg ggaggggcag gagcaggagg ggcaggagca ggaggagggg
6241 caggagcagg aggaggggca ggaggggcag gaggggcagg aggggcagga gcaggaggag
6301 gggcaggagc aggaggaggg gcaggagggg caggaggggc aggagcagga ggaggggcag
6361 gagcaggagg aggggcagga ggggcaggag caggaggagg ggcaggaggg gcaggagggg
6421 caggagcagg aggaggggca ggagcaggag gaggggcagg aggggcagga gcaggaggag
6481 gggcaggagg ggcaggaggg gcaggagcag gaggaggggc aggagcagga ggggcaggag
6541 gggcaggagg ggcaggagca ggaggggcag gagcaggagg aggggcagga ggggcaggag
6601 gggcaggagc aggaggggca ggagcaggag gggcaggagc aggaggggca ggagcaggag
6661 gggcaggagg ggcaggagca ggaggggcag gaggggcagg agcaggaggg gcaggagggg
6721 caggagcagg aggaggggca ggaggggcag gagcaggagg aggggcagga ggggcaggag
6781 caggaggggc aggaggggca ggagcaggag gggcaggagg ggcaggagca ggaggggcag
6841 gaggggcagg agcaggagga ggggcaggag caggaggggc aggagcagga ggtggaggcc
6901 ggggtcgagg aggcagtgga ggccggggtc gaggaggtag tggaggccgg ggtcgaggag
6961 gtagtggagg ccgccggggt agaggacgtg aaagagccag ggggggaagt cgtgaaagag
7021 ccagggggag aggtcgtgga cgtggagaaa agaggcccag gagtcccagt agtcagtcat
7081 catcatccgg gtctccaccg cgcaggcccc ctccaggtag aaggccattt ttccaccctg
7141 taggggaagc cgattatttt gaataccacc aagaaggtgg cccagatggt gagcctgacg
7201 tgcccccggg agcgatagag cagggccccg cagatgaccc aggagaaggc ccaagcactg
7261 gaccccgggg tcagggtgat ggaggcaggc gcaaaaaagg agggtggttt ggaaagcatc
7321 gtggtcaagg aggttccaac ccgaaatttg agaacattgc agaaggttta agagctctcc
DEFINITION pCEP4
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..10410
/organism="pCEP4"
/mol_type="other DNA"
misc_feature 112..134
/label="pGEX_3_primer"
terminator 279..410
/label="SV40_PA_terminator"
misc_feature 367..386
/label="EBV_rev_primer"
promoter complement(474..555)
/label="CMV_promoter"
misc_feature complement(474..498)
/label="LNCX_primer"
misc_feature complement(481..500)
/label="pCEP_fwd_primer"
misc_feature complement(524..544)
/label="CMV_fwd_primer"
CDS 1305..2045
/label="ORF frame 3"
terminator complement(1566..1836)
/label="TK_PA_terminator"
CDS complement(1844..2869)
/label="ORF frame 3"
gene complement(1847..2854)
/label="hygroB"
/gene="hygroB"
CDS 2111..3481
/label="ORF frame 2"
promoter 2988..3136
/label="TATA_promoter"
gene 3143..3481
/label="tet (852 - 1190)"
/gene="tet (852 - 1190)"
CDS 3385..3996
/label="ORF frame 1"
gene complement(4430..5290)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(4430..5290)
/label="ORF frame 3"
promoter complement(5332..5360)
/label="AmpR_promoter"
CDS complement(5914..7128)
/label="ORF frame 1"
CDS 5916..7841
/label="ORF frame 3"
CDS 6031..7143
/label="ORF frame 1"
rep_origin 8145..10315
/label="EBV_origin"
ORIGIN
1 tctagagtcg accggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
61 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
121 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagccg gatcataatc
181 agccatacca catttgtaga ggttttactt gctttaaaaa acctccccac ctccccctga
241 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
301 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
361 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cggccttgcc
421 ggcctcgagc ggccgctagc aagcttgcta gcagctggta cccagcttct agagatctga
481 cggttcacta aacgagctct gcttatatag acctcccacc gtacacgcct accgcccatt
541 tgcgtcaacg gggcggggtt attacgacat tttggaaagt cccgttgatt ttggtgccaa
601 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
661 tatccacgcc cattggtgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
721 acgtagatgt actgccaagt aggaaagtcc cgtaaggtca tgtactgggc ataatgccag
781 gcgggccatt taccgtcatt gacgtcaata gggggcggac ttggcatatg atacacttga
841 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
901 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
961 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
1021 ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa
1081 catggcggtc atattggaca tgagccaata taaatgtaca tattatgata tagatacaac
1141 gtatgcaatg gccaatagcc aatattgatt tatgctatat aaccaatgac taatatggct
1201 aattgccaat attgattcaa tgtatagatc ttccatacct accagttctg cgcctgcagc
1261 aatgcaacaa cgttgcccgg atctgcgatg ataagctgtc aaacatgaga attggtcgac
1321 tagcttggca cgccagaaat ccgcgcggtg gtttttgggg gtcgggggtg tttggcagcc
1381 acagacgccc ggtgttcgtg tcgcgccagt acatgcggtc catgcccagg ccatccaaaa
1441 accatgggtc tgtctgctca gtccagtcgt ggaccagacc ccacgcaacg cccaaaataa
1501 taacccccac gaaccataaa ccattcccca tgggggaccc cgtccctaac ccacggggcc
1561 agtggctatg gcagggcctg ccgccccgac gttggctgcg agccctgggc cttcacccga
1621 acttgggggg tggggtgggg aaaaggaaga aacgcgggcg tattggcccc aatggggtct
1681 cggtggggta tcgacagagt gccagccctg ggaccgaacc ccgcgtttat gaacaaacga
1741 cccaacaccc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg
1801 gtattgtctc cttccgtgtt tcagttagcc tcccccatct cccctattcc tttgccctcg
1861 gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc
1921 agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga
1981 cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc
2041 tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg
2101 caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg
2161 gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc
2221 agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg
2281 cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag
2341 cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg
2401 gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc
2461 cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatggcct
2521 ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac
2581 cctgtgcacg gcgggagatg caataggtca ggctctcgct gaattcccca atgtcaagca
2641 cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga
2701 aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga
2761 aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt
2821 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tctcattgcc
2881 cgggatctgc ggcacgctgt tgacgctgtt aagcgggtcg ctgcagggtc gctcggtgtt
2941 cgaggccaca cgcgtcacct taatatgcga agtggacctg ggaccgcgcc gccccgactg
3001 catctgcgtg ttcgaattcg ccaatgacaa gacgctgggc ggggtttgtg tcatcataga
3061 actaaagaca tgcaaatata tttcttccgg ggacaccgcc agcaaacgcg agcaacgggc
3121 cacggggatg aagcagggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc
3181 gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat
3241 gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca
3301 aggatcgctc gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc
3361 gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct
3421 ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg
3481 aatggaagcc ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa
3541 ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc
3601 gccatctcca gcagccgcac gcggcgcagc aaaaggccag gaaccgtaaa aaggccgcgt
3661 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
3721 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
3781 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
3841 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
3901 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
3961 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
4021 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
4081 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
4141 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
4201 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
4261 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
4321 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
4381 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
4441 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
4501 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
4561 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
4621 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
4681 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
4741 ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
4801 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
4861 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
4921 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
4981 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
5041 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
5101 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
5161 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
5221 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
5281 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
5341 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
5401 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
5461 aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt ctcatgtttg acagcttatc
5521 atcgataagc tgatcctcac aggccgcacc cagcttttct tccgttgccc cagtagcatc
5581 tctgtctggt gaccttgaag aggaagagga ggggtcccga gaatccccat ccctaccgtc
5641 cagcaaaaag ggggacgagg aatttgaggc ctggcttgag gctcaggacg caaatcttga
5701 ggatgttcag cgggagtttt ccgggctgcg agtaattggt gatgaggacg aggatggttc
5761 ggaggatggg gaattttcag acctggatct gtctgacagc gaccatgaag gggatgaggg
5821 tgggggggct gttggagggg gcaggagtct gcactccctg tattcactga gcgtcgtcta
5881 ataaagatgt ctattgatct cttttagtgt gaatcatgtc tgacgagggg ccaggtacag
5941 gacctggaaa tggcctagga gagaagggag acacatctgg accagaaggc tccggcggca
6001 gtggacctca aagaagaggg ggtgataacc atggacgagg acggggaaga ggacgaggac
6061 gaggaggcgg aagaccagga gccccgggcg gctcaggatc agggccaaga catagagatg
6121 gtgtccggag accccaaaaa cgtccaagtt gcattggctg caaagggacc cacggtggaa
6181 caggagcagg agcaggagcg ggaggggcag gagcaggagg ggcaggagca ggaggagggg
6241 caggagcagg aggaggggca ggaggggcag gaggggcagg aggggcagga gcaggaggag
6301 gggcaggagc aggaggaggg gcaggagggg caggaggggc aggagcagga ggaggggcag
6361 gagcaggagg aggggcagga ggggcaggag caggaggagg ggcaggaggg gcaggagggg
6421 caggagcagg aggaggggca ggagcaggag gaggggcagg aggggcagga gcaggaggag
6481 gggcaggagg ggcaggaggg gcaggagcag gaggaggggc aggagcagga ggggcaggag
6541 gggcaggagg ggcaggagca ggaggggcag gagcaggagg aggggcagga ggggcaggag
6601 gggcaggagc aggaggggca ggagcaggag gggcaggagc aggaggggca ggagcaggag
6661 gggcaggagg ggcaggagca ggaggggcag gaggggcagg agcaggaggg gcaggagggg
6721 caggagcagg aggaggggca ggaggggcag gagcaggagg aggggcagga ggggcaggag
6781 caggaggggc aggaggggca ggagcaggag gggcaggagg ggcaggagca ggaggggcag
6841 gaggggcagg agcaggagga ggggcaggag caggaggggc aggagcagga ggtggaggcc
6901 ggggtcgagg aggcagtgga ggccggggtc gaggaggtag tggaggccgg ggtcgaggag
6961 gtagtggagg ccgccggggt agaggacgtg aaagagccag ggggggaagt cgtgaaagag
7021 ccagggggag aggtcgtgga cgtggagaaa agaggcccag gagtcccagt agtcagtcat
7081 catcatccgg gtctccaccg cgcaggcccc ctccaggtag aaggccattt ttccaccctg
7141 taggggaagc cgattatttt gaataccacc aagaaggtgg cccagatggt gagcctgacg
7201 tgcccccggg agcgatagag cagggccccg cagatgaccc aggagaaggc ccaagcactg
7261 gaccccgggg tcagggtgat ggaggcaggc gcaaaaaagg agggtggttt ggaaagcatc
7321 gtggtcaagg aggttccaac ccgaaatttg agaacattgc agaaggttta agagctctcc
7381 tggctaggag tcacgtagaa aggactaccg acgaaggaac
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