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- 聯系人：Dr.Xu, Biovector NTCC Inc.
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手機：18901268599
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pCMVHA載體基本信息
| Clontech |
| pCMV-HA |
| 哺乳細胞表達系統載體 |
| 高拷貝 |
| CMV |
| 多克隆位點，限制性內切酶 |
| 3782bp |
| pCMV-F: 5'd[GATCCGGTACTAGAGGAACTGAAAAAC]3' |
| -- |
| HA (N term) |
| Ampicillin |
| -- |
| 用于在哺乳細胞中表達N端帶有HA標簽的蛋白 |
| 631604 |
| 瞬表達 |
| 組成型 |
| 非病毒 |
pCMVHA載體質粒圖譜和多克隆位點信息
pCMVHA載體簡介
pCMVHA載體序列
DEFINITION pCMV-HA
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..3782
/organism="pCMV-HA"
/mol_type="other DNA"
misc_feature 31..318
/label="CAG_enhancer"
promoter 36..528
/label="CMV_immearly_promoter"
misc_feature 485..505
/label="CMV_fwd_primer"
promoter 486..555
/label="CMV_promoter"
promoter 498..617
/label="CMV2_promoter"
misc_feature 529..548
/label="pCEP_fwd_primer"
misc_feature 531..555
/label="LNCX_primer"
misc_feature 631..657
/label="pCMV_primer"
misc_feature 672..768
/label="modSV40_late_16s_int"
misc_feature 672..702
/label="SV40_late_19s_int"
terminator complement(931..1050)
/label="SV40_PA_terminator"
misc_feature complement(945..964)
/label="EBV_rev_primer"
promoter complement(1162..1180)
/label="M13_reverse_primer"
misc_feature complement(1179..1201)
/label="M13_pUC_rev_primer"
promoter complement(1215..1244)
/label="lac_promoter"
gene complement(2327..3187)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(2327..3187)
/label="ORF frame 2"
promoter complement(3229..3257)
/label="AmpR_promoter"
misc_feature complement(3416..3438)
/label="pGEX_3_primer"
misc_feature complement(3637..3780)
/label="lacZ_a"
misc_feature 3751..3773
/label="M13_pUC_fwd_primer"
promoter 3766..3782
/label="M13_forward20_primer"
ORIGIN
1 GAGTTCGAGC TTGCATGCCT GCAGGTCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC
61 TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG
121 CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG
181 GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA
241 TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC
301 ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG
361 CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG
421 AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA
481 TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA
541 GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGACAC
601 CGGGACCGAT CCAGCCTCCG GACTCTAGAG GATCCGGTAC TAGAGGAACT GAAAAACCAG
661 AAAGTTAACT GGTAAGTTTA GTCTTTTTGT CTTTTATTTC AGGTCCCGGA TCCGGTGGTG
721 GTGCAAATCA AAGAACTGCT CCTCAGTGGA TGTTGCCTTT ACTTCTAGGC CTGTACGGAA
781 GTGTTACTTC TGCTCTAAAA GCTGCGGAAT TGTACCCGCG GGCCCACCAT GTACCCATAC
841 GATGTTCCAG ATTACGCTCT TATGGCCATG GAGGCCCGAA TTCGGTCGAC CGAGATCTCT
901 CGAGGTACCG CGGCCGCGGG GATCCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA
961 CCACAACTAG AATGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT
1021 TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA
1081 TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTCGGA TCCTCTAGAG TCGATCTGCA
1141 GGCATGCTAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC
1201 TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT
1261 GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC
1321 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG
1381 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG
1441 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG
1501 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC
1561 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC
1621 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC
1681 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT
1741 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG
1801 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT
1861 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG
1921 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT
1981 GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC
2041 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA
2101 GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG
2161 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA
2221 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA
2281 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA
2341 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC
2401 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA
2461 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA
2521 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT
2581 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG
2641 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC
2701 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG
2761 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG
2821 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT
2881 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT
2941 CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC
3001 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC
3061 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG
3121 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA
3181 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA
3241 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC
3301 CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA ACCTATAAAA
3361 ATAGGCGTAT CACGAGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT
3421 GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC
3481 AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG
3541 CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG CACAGATGCG
3601 TAAGGAGAAA ATACCGCATC AGGCGCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG
3661 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA
3721 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA
3781 GT
//
pCMVHA載體質粒圖譜pdf版和相關資料下載
pCMVHA載體應用舉例
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電郵Biovector@163.com
電話18901268599
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標簽：以下資源由Biovector Inc. 質粒載體菌株細胞株基因保藏中心獨家供應并發布，侵權必究。Biovector,Inc質粒載體菌株細胞株基因保藏中心列表網址http://biovector.blog.163.com 質粒 載體 索取 報價 價格 優惠 售價 出售 圖譜 序列 酶切位點 轉化 構建 求購 購買 訂購 sequence map plasmid vector gene strain order ship 抗性 resistance restriction sitepmCherry-C1
p3xFLAG-KV2.1
pGL3-control
pEGFP-1
pDsRed2-N1
pDsRed2-C1
pEF1/V5-HisA
pEF1/myc-His B
pcDNA4/TO
pcDNA4/V5-His A
pcDNA4/HisMax B
pEF6/myc-His C
pEF4/V5-His A
SuperCos1
pDsRED-Monomer-N1
pmCherry-N1
pDsRed2-1
pAcGFP1-1
pLEGFP-C1
pDsRed-Monomer
pEBFP-N1
pGluc-basic
psiCHECK 2
pRNA-H1.1/Adeno
pRNATin-H1.2/Neo
pRNATin-H1.2/Retro
pRNATin-H1.2/Hygro
pEYFP-N1
pECFP-N1
pECFP-C1
pSecTag2 A
pCMV-Tag 3B
pEGFP-N3
pBudCE4.1
pIRESneo3
pcDNA3.1/Myc-His B
pcDNA3.1/Myc-His C
pcDNA3.1/Myc-His A
pcDNA3.1/His C
pcDNA3.1/His B
pVitro2-neo-mcs
pFLAG-CMV2
pCMV-HA
pCMV-myc
pCAGGS
pSG5
pCMV-Tag2 B
pCMV-Tag 5B
pSFV1
pcDNA3.1(+)/Myc HisB
pUB6/V5 His B
pUB6/V5 His A
pUB6/V5 His C
pRL-TK
pNeoEGFP
c-Flag pcDNA3
pcDNA3.1/Hygro(-)
pcDNA6-Myc/His B
pCMV6-Entry
pCMV6-XL4
p3xFLAG-KV4.2
p3xFLAG-KV1.5
p3xFLAG-CMV-10
pIRES
pIRES-EGFP
pCEP4
pGL3 basic
pcDNA3.1(-)
pEGFP-C3
pGL3-Promoter
pcDNA3.1-EGFP
pcDNA3.1(+)
pGCSi/H1/Neo ShGFP
pDsRED2-Mito
pMIR-Report Luciferase
pIRES2-EGFP
pEGFP-C1
pEGFP-N1
PiggyBac Dual promoter
Super PiggyBac Transposase
pBC1
pDsRED2-Nuc
pDsRED2-Peroxi
pEBFP-C1
pECFP-ER
pECFP-Mito
pEYFP-actin
pEYFP-Golgi
pEYFP-Mem
pEYFP-tub
pGL3-Enhancer
pRL-CMV
pRL-SV40
pOG44
pORF-lacZ
pORF-HSV1tk
pEGFP-Actin
pWHERE
pN3
pEGFP-C2
pEGFP-N2
p3xFlag-CMV-14
pIRES-hrGFP-1a
pcDNA3.1/GS
pcDNA3.1/Zeo(+)
pcDNA3.1/Zeo(-)
pIREShyg3
pIRESpuro3
pGenesil-1
pBK-CMV
pNTAP-B
pVAX1
pIRESneo
pIRESneo2
pSI
pCI
pcDNA3.1/Hygro(+)
pBI-CMV1
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