pMIR-Report Luciferase質(zhì)粒載體圖譜序列抗性說明書價(jià)格報(bào)價(jià)
- 價(jià) 格：￥0
- 貨 號(hào)：40322383655
- 產(chǎn) 地：北京
- BioVector NTCC典型培養(yǎng)物保藏中心
- 聯(lián)系人：Dr.Xu, Biovector NTCC Inc.
電話：400-800-2947 工作QQ:1843439339 (微信同號(hào))
郵件：Biovector@163.com
手機(jī)：18901268599
地址：北京
- 已注冊(cè)
NT-SIZE: 16px; FONT-WEIGHT: normal; PADDING-TOP: 0px">載體基本信息
| Ambion |
| pMIR-Report Luciferase |
| 哺乳動(dòng)物細(xì)胞miRNA表達(dá)報(bào)告載體，熒光報(bào)告載體 |
| 高拷貝 |
| CMV |
| 多克隆位點(diǎn)，限制性內(nèi)切酶 |
| 6470bp |
| Luc-F: AGTCAAGTAACAACCGCGA; |
| -- |
| N-Luciferase(熒光素酶) |
| 氨芐抗性 |
| 嘌呤霉素 |
| 本載體是用來克隆插入miRNA靶序列，評(píng)估細(xì)胞內(nèi)miRNA功能的載體。 |
| AM5795 |
| 瞬時(shí)表達(dá), 穩(wěn)定表達(dá)都可以 |
| 非組成型 |
| 非病毒 |
載體質(zhì)粒圖譜和多克隆位點(diǎn)信息
載體簡(jiǎn)介
pMIR-REPORT miRNA表達(dá)報(bào)告基因載體系統(tǒng)由一個(gè)實(shí)驗(yàn)螢火蟲熒光素酶報(bào)告載體和相關(guān)的β-半乳糖苷酶報(bào)告對(duì)照質(zhì)粒組成。通過在多克隆位點(diǎn)插入預(yù)測(cè)的miRNA靶序列， pMIR-REPORT熒光素酶報(bào)告miRNA表達(dá)報(bào)告載體可以被用來進(jìn)行準(zhǔn)確的，定量的，評(píng)估細(xì)胞內(nèi)miRNA的功能。它也可以被用來檢測(cè)siRNA的靶位點(diǎn)和分析3'端非編碼區(qū)序列對(duì)基因表達(dá)的影響。 pMIR-REPORT Luciferase載體包含一個(gè)CMV啟動(dòng)子和終止子控制下的螢火蟲熒光素酶報(bào)告基因。在熒光素酶基因的3'端非編碼區(qū)包含一個(gè)多克隆位點(diǎn)，用于插入的預(yù)測(cè)miRNA的結(jié)合靶序列或其他核苷酸序列。通過將預(yù)測(cè)的miRNA靶序列克隆插入到pMIR-REPORT載體，熒光素酶報(bào)告表達(dá)就收到調(diào)節(jié)。這模仿的miRNA靶序列的作用方式。
pMIR-REPORT β-gal質(zhì)粒是一個(gè)β-半乳糖苷酶報(bào)告質(zhì)粒，其被設(shè)計(jì)用于細(xì)胞轉(zhuǎn)染操作流程的標(biāo)準(zhǔn)化探索。 該對(duì)照質(zhì)粒表達(dá)的β-半乳糖苷酶，可以用來標(biāo)準(zhǔn)化由于細(xì)胞活力和轉(zhuǎn)染效率差異導(dǎo)致的細(xì)胞表達(dá)水平的多樣性。
作為附加的功能，pMIR-REPORT Luciferase和pMIR-REPORT β-gal分別含有嘌呤霉素和新霉素抗性基因，這可以用于選擇轉(zhuǎn)染成功的哺乳動(dòng)物細(xì)胞。研究需要的話，抗生素選擇可用于構(gòu)建pMIR-REPORT載體質(zhì)粒穩(wěn)表達(dá)細(xì)胞系。對(duì)于大多數(shù)應(yīng)用，瞬表達(dá)是足夠的，抗生素的篩選也是沒有必要的。
載體序列
LOCUS pMIR-Report\Luciferase 6470 bp DNA SYN
DEFINITION pMIR-report
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..6470
/organism="pMIR-report"
/mol_type="other DNA"
misc_feature complement(29..51)
/label="pGEX_3_primer"
misc_feature complement(250..393)
/label="lacZ_a"
misc_feature 364..386
/label="M13_pUC_fwd_primer"
promoter 379..395
/label="M13_forward20_primer"
CDS complement(542..2269)
/label="ORF frame 2"
gene complement(560..2200)
/label="firefly_Luciferase (variant)"
/gene="firefly_Luciferase (variant)"
misc_feature 2116..2134
/label="LucNrev_primer"
promoter complement(2227..2791)
/label="CMV_immearly_promoter"
misc_feature complement(2254..2274)
/label="CMV_fwd_primer"
misc_feature complement(2440..2727)
/label="CAG_enhancer"
promoter complement(2854..2872)
/label="T7_promoter"
misc_feature complement(2885..2902)
/label="Sp6_primer"
misc_feature complement(2935..2957)
/label="M13_pUC_rev_primer"
promoter complement(2971..3000)
/label="lac_promoter"
gene complement(4083..4943)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(4083..4943)
/label="ORF frame 1"
promoter complement(4985..5013)
/label="AmpR_promoter"
CDS 5396..6139
/label="ORF frame 2"
CDS complement(5447..6046)
/label="ORF frame 2"
promoter complement(6191..6459)
/label="SV40_promoter"
misc_feature complement(6211..6230)
/label="SV40pro_F_primer"
rep_origin complement(6215..6292)
/label="SV40_origin"
misc_feature 6262..6453
/label="SV40_enhancer"
ORIGIN
1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC
241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT
301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT
361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGCCAA GCTAGCGGCC GCATACAAAA
421 AACCAACACA CAGATCCAAT GAAAATAAAA GATCCTTTAT TAAGCTTAAC TGTTTAAACC
481 TATGATATCA CGCACGCGTC TATGCCGGCC TATGAGCTCC CTCACTAGTG CGCAGCTTTC
541 ATCACTCGAG CAATTTGGAC TTTCCGCCCT TCTTGGCCTT TATGAGGATC TCTCTGATTT
601 TTCTTGCGTC GAGTTTTCCG GTAAGACCTT TCGGTACTTC GTCCACAAAC ACAACTCCTC
661 CGCGCAACTT TTTCGCGGTT GTTACTTGAC TGGCGACGTA ATCCACGATC TCTTTTTCCG
721 TCATCGTCTT TCCGTGCTCC AAAACAACAA CGGCGGCGGG AAGTTCACCG GCGTCATCGT
781 CGGGAAGACC TGCCACGCCC GCGTCGAAGA TGTTGGGGTG TTGTAACAAT ATCGATTCCA
841 ATTCAGCGGG GGCCACCTGA TATCCTTTGT ATTTAATTAA AGACTTCAAG CGGTCAACTA
901 TGAAGAAGTG TTCGTCTTCG TCCCAGTAAG CTATGTCTCC AGAATGTAGC CATCCATCCT
961 TGTCAATCAA GGCGTTGGTC GCTTCCGGAT TGTTTACATA ACCGGACATA ATCATAGGTC
1021 CTCTGACACA TAATTCGCCT CTCTGATTAA CGCCCAGCGT TTTCCCGGTA TCCAGATCCA
1081 CAACCTTCGC TTCAAAAAAT GGAACAACTT TACCGACCGC GCCCGGTTTA TCATCCCCCT
1141 CGGGTGTAAT CAGAATAGCT GATGTAGTCT CAGTGAGCCC ATATCCTTGT CGTATCCCTG
1201 GAAGATGGAA GCGTTTTGCA ACCGCTTCCC CGACTTCTTT CGAAAGAGGT GCGCCCCCAG
1261 AAGCAATTTC GTGTAAATTA GATAAATCGT ATTTGTCAAT CAGAGTGCTT TTGGCGAAGA
1321 ATGAAAATAG GGTTGGTACT AGCAACGCAC TTTGAATTTT GTAATCCTGA AGGGATCGTA
1381 AAAACAGCTC TTCTTCAAAT CTATACATTA AGACGACTCG AAATCCACAT ATCAAATATC
1441 CGAGTGTAGT AAACATTCCA AAACCGTGAT GGAATGGGAC AACACTTAAA ATCGCAGTAT
1501 CCGGAACGAT TTGATTGCCA AAAATAGGAT CTCTGGCATG CGAGAATCTG ACGCAGGCAG
1561 TTCTATGCGG AAGGGCCACA CCCTTAGGTA ACCCAGTAGA TCCAGAGGAA TTCATTATCA
1621 GTGCAATTGT TTTGTCACGA TCAAAGGACT CTGGTACAAA ATCGTATTCA TTAAAACCGG
1681 GAGGTAGATG AGATGTGACG AACGTGTACA TCGACTGAAA TCCCTGGTAA TCCGTTTTAG
1741 AATCCATGAT AATAATTTTC TGGATTATTG GTAATTTTTT TTGCACGTTC AAAATTTTTT
1801 GCAACCCCTT TTTGGAAACA AACACTACGG TAGGCTGCGA AATGTTCATA CTGTTGAGCA
1861 ATTCACGTTC ATTATAAATG TCGTTCGCGG GCGCAACTGC AACTCCGATA AATAACGCGC
1921 CCAACACCGG CATAAAGAAT TGAAGAGAGT TTTCACTGCA TACGACGATT CTGTGATTTG
1981 TATTCAGCCC ATATCGTTTC ATAGCTTCTG CCAACCGAAC GGACATTTCG AAGTATTCCG
2041 CGTACGTGAT GTTCACCTCG ATATGTGCAT CTGTAAAAGC AATTGTTCCA GGAACCAGGG
2101 CGTATCTCTT CATAGCCTTA TGCAGTTGCT CTCCAGCGGT TCCATCCTCT AGAGGATAGA
2161 ATGGCGCCGG GCCTTTCTTT ATGTTTTTGG CGTCTTCCAT GGTGGATCCA CGGTTCACTA
2221 AACCAGCTCT GCTTATATAG ACCTCCCACC GTACACGCCT ACCGCCCATT TGCGTCAATG
2281 GGGCGGAGTT GTTACGACAT TTTGGAAAGT CCCGTTGATT TTGGTGCCAA AACAAACTCC
2341 CATTGACGTC AATGGGGTGG AGACTTGGAA ATCCCCGTGA GTCAAACCGC TATCCACGCC
2401 CATTGATGTA CTGCCAAAAC CGCATCACCA TGGTAATAGC GATGACTAAT ACGTAGATGT
2461 ACTGCCAAGT AGGAAAGTCC CATAAGGTCA TGTACTGGGC ATAATGCCAG GCGGGCCATT
2521 TACCGTCATT GACGTCAATA GGGGGCGTAC TTGGCATATG ATACACTTGA TGTACTGCCA
2581 AGTGGGCAGT TTACCGTAAA TACTCCACCC ATTGACGTCA ATGGAAAGTC CCTATTGGCG
2641 TTACTATGGG AACATACGTC ATTATTGACG TCAATGGGCG GGGGTCGTTG GGCGGTCAGC
2701 CAGGCGGGCC ATTTACCGTA AGTTATGTAA CGCGGAACTC CATATATGGG CTATGAACTA
2761 ATGACCCCGT AATTGATTAC TATTAATAAC TAAGATCTGG TACCTTGAAT TCATGCTTCT
2821 CCTCCCTTTA GTGAGGGTAA TTCTCTCTCT CTCCCTATAG TGAGTCGTAT TAATTCCTTC
2881 TCTTCTATAG TGTCACCTAA ATCGTTGCAA TTCGTAATCA TGTCATAGCT GTTTCCTGTG
2941 TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA
3001 GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT
3061 TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA
3121 GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC
3181 GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA
3241 TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT
3301 AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA
3361 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT
3421 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG
3481 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC
3541 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC
3601 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA
3661 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT
3721 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC
3781 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA
3841 AAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA
3901 AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA
3961 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT
4021 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC
4081 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC
4141 ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC
4201 CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA
4261 AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC
4321 CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC
4381 AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA
4441 TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA
4501 GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA
4561 CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT
4621 TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT
4681 TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG
4741 CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA
4801 TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC
4861 AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG
4921 ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG
4981 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG
5041 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG
5101 ACATTAACCT ATAAAAATAG GCGTATCACG AGATTGCAGT GAAAAAAATG CTTTATTTGT
5161 GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC
5221 AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA
5281 AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG ATCCTCTAGA GTCGGTGGGC
5341 CTCGGGGGCG GGTGCGGGGT CGGCGGGGCC GCCCCGGGTG GCTTCGGTCG GAGCCATGGG
5401 GTCGTGCGCT CCTTTCGGTC GGGCGCTGCG GGTCGTGGGG CGGGCGTCAG GCACCGGGCT
5461 TGCGGGTCAT GCACCAGGTG CGCGGTCCTT CGGGCACCTC GACGTCGGCG GTGACGGTGA
5521 AGCCGAGCCG CTCGTAGAAG GGGAGGTTGC GGGGCGCGGA GGTCTCCAGG AAGGCGGGCA
5581 CCCCGGCGCG CTCGGCCGCC TCCACTCCGG GGAGCACGAC GGCGCTGCCC AGACCCTTGC
5641 CCTGGTGGTC GGGCGAGACG CCGACGGTGG CCAGGAACCA CGCGGGCTCC TTGGGCCGGT
5701 GCGGCGCCAG GAGGCCTTCC ATCTGTTGCT GCGCGGCCAG CCGGGAACCG CTCAACTCGG
5761 CCATGCGCGG GCCGATCTCG GCGAACACCG CCCCCGCTTC GACGCTCTCC GGCGTGGTCC
5821 AGACCGCCAC CGCGGCGCCG TCGTCCGCGA CCCACACCTT GCCGATGTCG AGCCCGACGC
5881 GCGTGAGGAA GAGTTCTTGC AGCTCGGTGA CCCGCTCGAT GTGGCGGTCC GGGTCGACGG
5941 TGTGGCGCGT GGCGGGGTAG TCGGCGAACG CGGCGGCGAG GGTGCGTACG GCCCGGGGGA
6001 CGTCGTCGCG GGTGGCGAGG CGCACCGTGG GCTTGTACTC GGTCATGGAA GGTCGTCTCC
6061 TTGTGAGGGG TCAGGGGCGT GGGTCAGGGG ATGGTGGCGG CACCGGTCGT GGCGGCCGAC
6121 CTGCAGGCAT GCAAGCTAGC TTTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT
6181 ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA
6241 GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG
6301 TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG
6361 CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA
6421 TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCA ATCTTTCGTC
//
DEFINITION pMIR-report
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..6470
/organism="pMIR-report"
/mol_type="other DNA"
misc_feature complement(29..51)
/label="pGEX_3_primer"
misc_feature complement(250..393)
/label="lacZ_a"
misc_feature 364..386
/label="M13_pUC_fwd_primer"
promoter 379..395
/label="M13_forward20_primer"
CDS complement(542..2269)
/label="ORF frame 2"
gene complement(560..2200)
/label="firefly_Luciferase (variant)"
/gene="firefly_Luciferase (variant)"
misc_feature 2116..2134
/label="LucNrev_primer"
promoter complement(2227..2791)
/label="CMV_immearly_promoter"
misc_feature complement(2254..2274)
/label="CMV_fwd_primer"
misc_feature complement(2440..2727)
/label="CAG_enhancer"
promoter complement(2854..2872)
/label="T7_promoter"
misc_feature complement(2885..2902)
/label="Sp6_primer"
misc_feature complement(2935..2957)
/label="M13_pUC_rev_primer"
promoter complement(2971..3000)
/label="lac_promoter"
gene complement(4083..4943)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(4083..4943)
/label="ORF frame 1"
promoter complement(4985..5013)
/label="AmpR_promoter"
CDS 5396..6139
/label="ORF frame 2"
CDS complement(5447..6046)
/label="ORF frame 2"
promoter complement(6191..6459)
/label="SV40_promoter"
misc_feature complement(6211..6230)
/label="SV40pro_F_primer"
rep_origin complement(6215..6292)
/label="SV40_origin"
misc_feature 6262..6453
/label="SV40_enhancer"
ORIGIN
1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC
241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT
301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT
361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGCCAA GCTAGCGGCC GCATACAAAA
421 AACCAACACA CAGATCCAAT GAAAATAAAA GATCCTTTAT TAAGCTTAAC TGTTTAAACC
481 TATGATATCA CGCACGCGTC TATGCCGGCC TATGAGCTCC CTCACTAGTG CGCAGCTTTC
541 ATCACTCGAG CAATTTGGAC TTTCCGCCCT TCTTGGCCTT TATGAGGATC TCTCTGATTT
601 TTCTTGCGTC GAGTTTTCCG GTAAGACCTT TCGGTACTTC GTCCACAAAC ACAACTCCTC
661 CGCGCAACTT TTTCGCGGTT GTTACTTGAC TGGCGACGTA ATCCACGATC TCTTTTTCCG
721 TCATCGTCTT TCCGTGCTCC AAAACAACAA CGGCGGCGGG AAGTTCACCG GCGTCATCGT
781 CGGGAAGACC TGCCACGCCC GCGTCGAAGA TGTTGGGGTG TTGTAACAAT ATCGATTCCA
841 ATTCAGCGGG GGCCACCTGA TATCCTTTGT ATTTAATTAA AGACTTCAAG CGGTCAACTA
901 TGAAGAAGTG TTCGTCTTCG TCCCAGTAAG CTATGTCTCC AGAATGTAGC CATCCATCCT
961 TGTCAATCAA GGCGTTGGTC GCTTCCGGAT TGTTTACATA ACCGGACATA ATCATAGGTC
1021 CTCTGACACA TAATTCGCCT CTCTGATTAA CGCCCAGCGT TTTCCCGGTA TCCAGATCCA
1081 CAACCTTCGC TTCAAAAAAT GGAACAACTT TACCGACCGC GCCCGGTTTA TCATCCCCCT
1141 CGGGTGTAAT CAGAATAGCT GATGTAGTCT CAGTGAGCCC ATATCCTTGT CGTATCCCTG
1201 GAAGATGGAA GCGTTTTGCA ACCGCTTCCC CGACTTCTTT CGAAAGAGGT GCGCCCCCAG
1261 AAGCAATTTC GTGTAAATTA GATAAATCGT ATTTGTCAAT CAGAGTGCTT TTGGCGAAGA
1321 ATGAAAATAG GGTTGGTACT AGCAACGCAC TTTGAATTTT GTAATCCTGA AGGGATCGTA
1381 AAAACAGCTC TTCTTCAAAT CTATACATTA AGACGACTCG AAATCCACAT ATCAAATATC
1441 CGAGTGTAGT AAACATTCCA AAACCGTGAT GGAATGGGAC AACACTTAAA ATCGCAGTAT
1501 CCGGAACGAT TTGATTGCCA AAAATAGGAT CTCTGGCATG CGAGAATCTG ACGCAGGCAG
1561 TTCTATGCGG AAGGGCCACA CCCTTAGGTA ACCCAGTAGA TCCAGAGGAA TTCATTATCA
1621 GTGCAATTGT TTTGTCACGA TCAAAGGACT CTGGTACAAA ATCGTATTCA TTAAAACCGG
1681 GAGGTAGATG AGATGTGACG AACGTGTACA TCGACTGAAA TCCCTGGTAA TCCGTTTTAG
1741 AATCCATGAT AATAATTTTC TGGATTATTG GTAATTTTTT TTGCACGTTC AAAATTTTTT
1801 GCAACCCCTT TTTGGAAACA AACACTACGG TAGGCTGCGA AATGTTCATA CTGTTGAGCA
1861 ATTCACGTTC ATTATAAATG TCGTTCGCGG GCGCAACTGC AACTCCGATA AATAACGCGC
1921 CCAACACCGG CATAAAGAAT TGAAGAGAGT TTTCACTGCA TACGACGATT CTGTGATTTG
1981 TATTCAGCCC ATATCGTTTC ATAGCTTCTG CCAACCGAAC GGACATTTCG AAGTATTCCG
2041 CGTACGTGAT GTTCACCTCG ATATGTGCAT CTGTAAAAGC AATTGTTCCA GGAACCAGGG
2101 CGTATCTCTT CATAGCCTTA TGCAGTTGCT CTCCAGCGGT TCCATCCTCT AGAGGATAGA
2161 ATGGCGCCGG GCCTTTCTTT ATGTTTTTGG CGTCTTCCAT GGTGGATCCA CGGTTCACTA
2221 AACCAGCTCT GCTTATATAG ACCTCCCACC GTACACGCCT ACCGCCCATT TGCGTCAATG
2281 GGGCGGAGTT GTTACGACAT TTTGGAAAGT CCCGTTGATT TTGGTGCCAA AACAAACTCC
2341 CATTGACGTC AATGGGGTGG AGACTTGGAA ATCCCCGTGA GTCAAACCGC TATCCACGCC
2401 CATTGATGTA CTGCCAAAAC CGCATCACCA TGGTAATAGC GATGACTAAT ACGTAGATGT
2461 ACTGCCAAGT AGGAAAGTCC CATAAGGTCA TGTACTGGGC ATAATGCCAG GCGGGCCATT
2521 TACCGTCATT GACGTCAATA GGGGGCGTAC TTGGCATATG ATACACTTGA TGTACTGCCA
2581 AGTGGGCAGT TTACCGTAAA TACTCCACCC ATTGACGTCA ATGGAAAGTC CCTATTGGCG
2641 TTACTATGGG AACATACGTC ATTATTGACG TCAATGGGCG GGGGTCGTTG GGCGGTCAGC
2701 CAGGCGGGCC ATTTACCGTA AGTTATGTAA CGCGGAACTC CATATATGGG CTATGAACTA
2761 ATGACCCCGT AATTGATTAC TATTAATAAC TAAGATCTGG TACCTTGAAT TCATGCTTCT
2821 CCTCCCTTTA GTGAGGGTAA TTCTCTCTCT CTCCCTATAG TGAGTCGTAT TAATTCCTTC
2881 TCTTCTATAG TGTCACCTAA ATCGTTGCAA TTCGTAATCA TGTCATAGCT GTTTCCTGTG
2941 TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA
3001 GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT
3061 TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA
3121 GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC
3181 GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA
3241 TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT
3301 AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA
3361 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT
3421 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG
3481 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC
3541 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC
3601 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA
3661 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT
3721 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC
3781 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA
3841 AAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA
3901 AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA
3961 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT
4021 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC
4081 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC
4141 ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC
4201 CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA
4261 AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC
4321 CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC
4381 AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA
4441 TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA
4501 GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA
4561 CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT
4621 TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT
4681 TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG
4741 CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA
4801 TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC
4861 AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG
4921 ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG
4981 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG
5041 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG
5101 ACATTAACCT ATAAAAATAG GCGTATCACG AGATTGCAGT GAAAAAAATG CTTTATTTGT
5161 GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC
5221 AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA
5281 AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG ATCCTCTAGA GTCGGTGGGC
5341 CTCGGGGGCG GGTGCGGGGT CGGCGGGGCC GCCCCGGGTG GCTTCGGTCG GAGCCATGGG
5401 GTCGTGCGCT CCTTTCGGTC GGGCGCTGCG GGTCGTGGGG CGGGCGTCAG GCACCGGGCT
5461 TGCGGGTCAT GCACCAGGTG CGCGGTCCTT CGGGCACCTC GACGTCGGCG GTGACGGTGA
5521 AGCCGAGCCG CTCGTAGAAG GGGAGGTTGC GGGGCGCGGA GGTCTCCAGG AAGGCGGGCA
5581 CCCCGGCGCG CTCGGCCGCC TCCACTCCGG GGAGCACGAC GGCGCTGCCC AGACCCTTGC
5641 CCTGGTGGTC GGGCGAGACG CCGACGGTGG CCAGGAACCA CGCGGGCTCC TTGGGCCGGT
5701 GCGGCGCCAG GAGGCCTTCC ATCTGTTGCT GCGCGGCCAG CCGGGAACCG CTCAACTCGG
5761 CCATGCGCGG GCCGATCTCG GCGAACACCG CCCCCGCTTC GACGCTCTCC GGCGTGGTCC
5821 AGACCGCCAC CGCGGCGCCG TCGTCCGCGA CCCACACCTT GCCGATGTCG AGCCCGACGC
5881 GCGTGAGGAA GAGTTCTTGC AGCTCGGTGA CCCGCTCGAT GTGGCGGTCC GGGTCGACGG
5941 TGTGGCGCGT GGCGGGGTAG TCGGCGAACG CGGCGGCGAG GGTGCGTACG GCCCGGGGGA
6001 CGTCGTCGCG GGTGGCGAGG CGCACCGTGG GCTTGTACTC GGTCATGGAA GGTCGTCTCC
6061 TTGTGAGGGG TCAGGGGCGT GGGTCAGGGG ATGGTGGCGG CACCGGTCGT GGCGGCCGAC
6121 CTGCAGGCAT GCAAGCTAGC TTTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT
6181 ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA
6241 GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG
6301 TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG
6361 CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA
6421 TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCA ATCTTTCGTC
//
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