pGAPZ C質(zhì)粒載體圖譜序列抗性說明書價(jià)格Biovector公司
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標(biāo)pGAPZC載體基本信息
Invitrogen |
pGAPZ C, pGAPZ-C, pGAPZC |
畢赤酵母蛋白表達(dá)載體 |
高拷貝 |
GAP |
多克隆位點(diǎn),限制性內(nèi)切酶 |
2883 bp |
pGAP-F:5´-GTCCCTATTTCAATCAATTGAA-3´ |
3´ AOX1:5´-GCAAATGGCATTCTGACATCC-3´ |
C-Myc, C-His |
Zeocin 博來霉素 |
HIS4/ Zeocin |
插入基因是必需包含起始密碼子ATG。 |
V200-20 |
穩(wěn)定 Stable |
組成型 Constitutive |
非病毒 |
pGAPZC載體質(zhì)粒圖譜和多克隆位點(diǎn)信息
pGAPZC載體簡(jiǎn)介
甘油醛-3-磷酸脫氫酶(GAPDH)的酶在許多生物細(xì)胞中包括畢赤酵母,穩(wěn)定高水平表達(dá)。 最近,GAPDH蛋白編碼基因啟動(dòng)子(GAP)被深入研究,并在畢赤酵母中能夠高水表達(dá)重組蛋白,這取決于使用的碳源(Waterham等 人,,1997)。GAP啟動(dòng)子(PGAP)的重組蛋白表達(dá)水平略高于AOX1啟動(dòng)子。
pGAPZ A,B,和C載體(2.9 KB)和pGAPZα,B,和C(3.1 KB)載體使用GAP啟動(dòng)子在畢赤酵母中穩(wěn)定表達(dá)重組蛋白。重組蛋白表達(dá)為為帶有C-末端myc抗原表位和C端His標(biāo)簽的融合蛋白。此外,pGAPZα 表達(dá)的融合蛋白在N-端帶有釀酒酵母(Saccharomyces cerevisiae)的α-因子分泌信號(hào)肽。這兩個(gè)系列的載體都提供三個(gè)不同讀碼框的載體,以方便克隆的帶有C-端標(biāo)簽和/或N-末端分泌信號(hào)肽的載體 中。
這些載體是基于顯性選擇標(biāo)記,Zeocin(博萊霉素)的篩選標(biāo)記可以同時(shí)使用于畢赤酵母和大腸桿菌。
pGAPZC載體序列
DEFINITION Pichia pastoris vector for constitutive intracellular expression of
a protein.
ACCESSION .
VERSION .
KEYWORDS pGAPZ C
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT Linearize within the GAP promoter for integration.
FEATURES Location/Qualifiers
source 1..2883
/organism="synthetic DNA construct"
/lab_host="Pichia pastoris"
/mol_type="other DNA"
promoter 7..483
/note="GAP promoter"
/note="promoter for Pichia pastoris
glyceraldehyde-3-phosphate dehydrogenase"
/note="color: #ffffff; direction: RIGHT"
misc_feature 484..562
/note="MCS"
/note="multiple cloning site"
/note="color: #99ccff"
CDS 563..592
/codon_start=1
/product="Myc (human c-Myc oncogene) epitope tag"
/note="Myc"
/note="color: #cc99b2"
/translation="EQKLISEEDL"
CDS 608..625
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/note="color: #cc99b2"
/translation="HHHHHH"
terminator 704..950
/gene="Pichia pastoris AOX1"
/note="AOX1 terminator"
/note="transcription terminator for AOX1"
/note="color: #ffffff"
promoter 965..1376
/gene="S. cerevisiae TEF1"
/note="TEF1 promoter"
/note="promoter for?EF-1-alpha"
/note="color: #ffffff; direction: RIGHT"
promoter 1383..1430
/note="EM7 promoter"
/note="synthetic bacterial promoter?"
/note="color: #ffffff; direction: RIGHT"
CDS 1449..1823
/codon_start=1
/gene="Sh?ble from?Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/note="BleoR"
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/note="color: #ccffcc"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
terminator 1889..2136
/gene="S. cerevisiae CYC1"
/note="CYC1 terminator"
/note="transcription terminator for CYC1"
/note="color: #ffffff"
rep_origin complement(2211..2799)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
ORIGIN
1 agatcttttt tgtagaaatg tcttggtgtc ctcgtccaat caggtagcca tctctgaaat
61 atctggctcc gttgcaactc cgaacgacct gctggcaacg taaaattctc cggggtaaaa
121 cttaaatgtg gagtaatgga accagaaacg tctcttccct tctctctcct tccaccgccc
181 gttaccgtcc ctaggaaatt ttactctgct ggagagcttc ttctacggcc cccttgcagc
241 aatgctcttc ccagcattac gttgcgggta aaacggaggt cgtgtacccg acctagcagc
301 ccagggatgg aaaagtcccg gccgtcgctg gcaataatag cgggcggacg catgtcatga
361 gattattgga aaccaccaga atcgaatata aaaggcgaac acctttccca attttggttt
421 ctcctgaccc aaagacttta aatttaattt atttgtccct atttcaatca attgaacaac
481 tatttcgaaa cgaggaattc acgtggccca gccggccgtc tcggatcggt acctcgagcc
541 gcggcggccg ccagcttacg tagaacaaaa actcatctca gaagaggatc tgaatagcgc
601 cgtcgaccat catcatcatc atcattgagt tttagcctta gacatgactg ttcctcagtt
661 caagttgggc acttacgaga agaccggtct tgctagattc taatcaagag gatgtcagaa
721 tgccatttgc ctgagagatg caggcttcat ttttgatact tttttatttg taacctatat
781 agtataggat tttttttgtc attttgtttc ttctcgtacg agcttgctcc tgatcagcct
841 atctcgcagc tgatgaatat cttgtggtag gggtttggga aaatcattcg agtttgatgt
901 ttttcttggt atttcccact cctcttcaga gtacagaaga ttaagtgaga ccttcgtttg
961 tgcggatccc ccacacacca tagcttcaaa atgtttctac tcctttttta ctcttccaga
1021 ttttctcgga ctccgcgcat cgccgtacca cttcaaaaca cccaagcaca gcatactaaa
1081 ttttccctct ttcttcctct agggtgtcgt taattacccg tactaaaggt ttggaaaaga
1141 aaaaagagac cgcctcgttt ctttttcttc gtcgaaaaag gcaataaaaa tttttatcac
1201 gtttcttttt cttgaaattt ttttttttag tttttttctc tttcagtgac ctccattgat
1261 atttaagtta ataaacggtc ttcaatttct caagtttcag tttcattttt cttgttctat
1321 tacaactttt tttacttctt gttcattaga aagaaagcat agcaatctaa tctaagggcg
1381 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
1441 actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
1501 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
1561 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
1621 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
1681 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
1741 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
1801 tcgtggccga ggagcaggac tgacacgtcc gacggcggcc cacgggtccc aggcctcgga
1861 gatccgtccc ccttttcctt tgtcgatatc atgtaattag ttatgtcacg cttacattca
1921 cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag
1981 gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt
2041 tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt
2101 gagaaggttt tgggacgctc gaaggcttta atttgcaagc tggagaccaa catgtgagca
2161 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
2221 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
2281 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
2341 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
2401 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
2461 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
2521 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
2581 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
2641 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
2701 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
2761 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
2821 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcatgag
2881 atc
//
pGAPZC載體質(zhì)粒圖譜pdf版和相關(guān)資料下載
pGAPZC載體應(yīng)用舉例
簽:以下資源由Biovector Inc. 質(zhì)粒載體菌株細(xì)胞株基因保藏中心獨(dú)家供應(yīng)并發(fā)布,侵權(quán)必究。Biovector,Inc質(zhì)粒載體菌株細(xì)胞株基因保藏中心列表網(wǎng)址http://biovector.blog.163.com 質(zhì)粒 載體 索取 報(bào)價(jià) 價(jià)格 優(yōu)惠 售價(jià) 出售 圖譜 序列 酶切位點(diǎn) 轉(zhuǎn)化 構(gòu)建 求購(gòu) 購(gòu)買 訂購(gòu) sequence map plasmid vector gene strain order ship 抗性 resistance restriction sitepYIP5
pDR195
pPICZα A
pHIC-PI
pRS426gal
pYEPlac112
pYX212
pFLD/CAT
pPICZα B
pESC-Leu
pYES2/CT
pYES2-EGFP
pMET C
pPink-LC
pGAPZα C
pGAPZ B
pPIC9K
pPIC9
SUMO protease
pGADT7
pYES2
pPIC ZαD
Ycplac33
p416GFD
pYRP7
pRS41H
pRS316
pESC-His
pYES2-NTC
pMETαC
pMET B
pPink-HC
pGAPZα B
pGAPZ A
pAO815
pPIC9k-His
pPICZ A
BFD15
pPIC ZαGB
pYEPlac195
pADH2
pYCP211
pFA6a-FGP(S65T)-kanMX6
pESC-URA
pYES6/CT
pYES2-NTA
pMETαB
pMET A
pHIL-D2
pGAPZα A
pPICZα C
pPIC3.5K
pAUR123
pPICZ C
LA503
pPIC ZαFC
Ycp22lac-EGFP
pYIP211
pYES2-kan
pRS426
pESC-TRP
pYES3/CT
pYES2-NTB
pMETαA
pPinkα-HC
pHIL-S1
pGAPZ C
pPICZ B
pPIC3.5
pSH47
pSH63
pSH65
pGBKT7
pHis2
pHisSi-1
pDest22
pGBKT7-53
pMyr
pGADT7-T
p53blue
pSos MAFB
pSos
p53his
pCL1
pGBKT7-Lam
pACT2 AD
pGAG424
pDest32
pBridge
pYD1
pDisplay
AH109
PichiaPink Strain3
SMD1168H
X-33
INVSC1
PichiaPink Strain2
SMD1163
KM71
SMD1168
PichiaPink Strain1
KM71H
PichiaPink Strain4
EBY100
GS115

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