pYES2-NTA質(zhì)粒載體圖譜序列抗性說明書價(jià)格Biovector公司
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| Invitrogen |
| pYES2-NTB, pYES2/NT B |
| 釀酒酵母蛋白表達(dá)載體 |
| 高拷貝 |
| 半乳糖 |
| GAL1 |
| 多克隆位點(diǎn)，限制性內(nèi)切酶 |
| 6039 bp |
| T7: TAATACGACTCACTATAGGG |
| CYC1 Terminator: GTGACATAACTAATTACATGATG |
| N-His, N-Xpress, C-His, C-V5 |
| 氨芐 |
| URA3 |
| 利用半乳糖誘導(dǎo)蛋白在釀酒酵母中表達(dá)。 |
| V8252-20 |
| 穩(wěn)定 Stable |
| 誘導(dǎo)型 |
| 非病毒 |
載體質(zhì)粒圖譜和多克隆位點(diǎn)信息
載體簡(jiǎn)介
pYES2 NT B的是一個(gè)6.0 kb的載體，設(shè)計(jì)用來在釀酒酵母（Saccharomyces cerevisiae）中誘導(dǎo)表達(dá)重組蛋白。
該載體包含以下元素：
1. 酵母GAL1啟動(dòng)子，能夠在釀酒酵母中被半乳糖高水平的誘導(dǎo)蛋白表達(dá)目的蛋白，同時(shí)能夠被葡萄糖抑制表達(dá)
2.多克隆位點(diǎn)可以使用的很多限制酶切位點(diǎn)，便于基因插入。
3.CYC1終止子能夠有效終止mRNA的轉(zhuǎn)錄。
4.能夠利用URA3基因篩選帶有ura3基因型的酵母宿主菌株轉(zhuǎn)化子。
5.氨芐抗性基因能夠方便在大腸桿菌中的進(jìn)行載體篩選。
6. 載體本身帶有N端His和Xpress標(biāo)簽，同時(shí)載體C端也帶有V5抗原表位和His純化標(biāo)簽。
8. 在酵母中，利用載體上的2μ復(fù)制子維持質(zhì)粒的高拷貝。
載體序列
LOCUS pYES2/NT\B 6039 bp DNA circular SYN
DEFINITION pYES2/NT B
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..6039
/organism="pYES2/NT B"
/mol_type="other DNA"
promoter 1..451
/label="GAL1_promoter"
misc_feature 414..437
/label="GAL1_primer"
promoter 475..493
/label="T7_promoter"
misc_feature 542..560
/label="Xpress_fwd_primer"
misc_feature 543..575
/label="T7_leader"
misc_feature 579..602
/label="Xpress_EK"
terminator 792..1037
/label="CYC1_terminator"
misc_feature complement(801..819)
/label="CYC1_primer"
misc_feature complement(801..819)
/label="pYESTrp_rev_primer"
rep_origin complement(1265..1884)
/label="pBR322_origin"
CDS complement(2039..2899)
/label="ORF frame 3"
CDS complement(2995..3798)
/label="ORF frame 1"
gene complement(2998..3798)
/label="URA3"
/gene="URA3"
CDS 3287..3763
/label="ORF frame 2"
promoter complement(3800..4025)
/label="URA3_promoter"
rep_origin 4028..5499
/label="2micron_origin"
ORIGIN
1 ACGGATTAGA AGCCGCCGAG CGGGTGACAG CCCTCCGAAG GAAGACTCTC CTCCGTGCGT
61 CCTCGTCTTC ACCGGTCGCG TTCCTGAAAC GCAGATGTGC CTCGCGCCGC ACTGCTCCGA
121 ACAATAAAGA TTCTACAATA CTAGCTTTTA TGGTTATGAA GAGGAAAAAT TGGCAGTAAC
181 CTGGCCCCAC AAACCTTCAA ATGAACGAAT CAAATTAACA ACCATAGGAT GATAATGCGA
241 TTAGTTTTTT AGCCTTATTT CTGGGGTAAT TAATCAGCGA AGCGATGATT TTTGATCTAT
301 TAACAGATAT ATAAATGCAA AAACTGCATA ACCACTTTAA CTAATACTTT CAACATTTTC
361 GGTTTGTATT ACTTCTTATT CAAATGTAAT AAAAGTATCA ACAAAAAATT GTTAATATAC
421 CTCTATACTT TAACGTCAAG GAGAAAAAAC CCCGGATCGG ACTACTAGCA GCTGTAATAC
481 GACTCACTAT AGGGAATATT AAGCTTACCA TGGGGGGTTC TCATCATCAT CATCATCATG
541 GTATGGCTAG CATGACTGGT GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA
601 AGGTACCTAA GGATCCAGTG TGGTGGAATT CTGCAGATAT CCAGCACAGT GGCGGCCGCT
661 CGAGTCTAGA GGGCCCTTCG AAGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC
721 TACGCGTACC GGTCATCATC ACCATCACCA TTGAGTTTAA ACCCGCTGAT CCTAGAGGGC
781 CGCATCATGT AATTAGTTAT GTCACGCTTA CATTCACGCC CTCCCCCCAC ATCCGCTCTA
841 ACCGAAAAGG AAGGAGTTAG ACAACCTGAA GTCTAGGTCC CTATTTATTT TTTTATAGTT
901 ATGTTAGTAT TAAGAACGTT ATTTATATTT CAAATTTTTC TTTTTTTTCT GTACAGACGC
961 GTGTACGCAT GTAACATTAT ACTGAAAACC TTGCTTGAGA AGGTTTTGGG ACGCTCGAAG
1021 GCTTTAATTT GCAAGCTGCG GCCCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG
1081 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC
1141 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG
1201 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGCCCAGG AACCGTAAAA
1261 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC
1321 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC
1381 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG
1441 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT
1501 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC
1561 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC
1621 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG
1681 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG
1741 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA
1801 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG
1861 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT
1921 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA
1981 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT
2041 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG
2101 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GCGCTTACCA TCTGGCCCCA
2161 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC
2221 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT
2281 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG
2341 TTGTTGGCAT TGCTACAGGC ATCGTGGTGT CACTCTCGTC GTTTGGTATG GCTTCATTCA
2401 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG
2461 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA
2521 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG
2581 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT
2641 CTTGCCCGGC GTCAATACGG GATAATAGTG TATCACATAG CAGAACTTTA AAAGTGCTCA
2701 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA
2761 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG
2821 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC
2881 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATGGGT AATAACTGAT ATAATTAAAT
2941 TGAAGCTCTA ATTTGTGAGT TTAGTATACA TGCATTTACT TATAATACAG TTTTTTAGTT
3001 TTGCTGGCCG CATCTTCTCA AATATGCTTC CCAGCCTGCT TTTCTGTAAC GTTCACCCTC
3061 TACCTTAGCA TCCCTTCCCT TTGCAAATAG TCCTCTTCCA ACAATAATAA TGTCAGATCC
3121 TGTAGAGACC ACATCATCCA CGGTTCTATA CTGTTGACCC AATGCGTCTC CCTTGTCATC
3181 TAAACCCACA CCGGGTGTCA TAATCAACCA ATCGTAACCT TCATCTCTTC CACCCATGTC
3241 TCTTTGAGCA ATAAAGCCGA TAACAAAATC TTTGTCGCTC TTCGCAATGT CAACAGTACC
3301 CTTAGTATAT TCTCCAGTAG ATAGGGAGCC CTTGCATGAC AATTCTGCTA ACATCAAAAG
3361 GCCTCTAGGT TCCTTTGTTA CTTCTTCTGC CGCCTGCTTC AAACCGCTAA CAATACCTGG
3421 GCCCACCACA CCGTGTGCAT TCGTAATGTC TGCCCATTCT GCTATTCTGT ATACACCCGC
3481 AGAGTACTGC AATTTGACTG TATTACCAAT GTCAGCAAAT TTTCTGTCTT CGAAGAGTAA
3541 AAAATTGTAC TTGGCGGATA ATGCCTTTAG CGGCTTAACT GTGCCCTCCA TGGAAAAATC
3601 AGTCAAGATA TCCACATGTG TTTTTAGTAA ACAAATTTTG GGACCTAATG CTTCAACTAA
3661 CTCCAGTAAT TCCTTGGTGG TACGAACATC CAATGAAGCA CACAAGTTTG TTTGCTTTTC
3721 GTGCATGATA TTAAATAGCT TGGCAGCAAC AGGACTAGGA TGAGTAGCAG CACGTTCCTT
3781 ATATGTAGCT TTCGACATGA TTTATCTTCG TTTCCTGCAG GTTTTTGTTC TGTGCAGTTG
3841 GGTTAAGAAT ACTGGGCAAT TTCATGTTTC TTCAACACTA CATATGCGTA TATATACCAA
3901 TCTAAGTCTG TGCTCCTTCC TTCGTTCTTC CTTCTGTTCG GAGATTACCG AATCAAAAAA
3961 ATTTCAAAGA AACCGAAATC AAAAAAAAGA ATAAAAAAAA AATGATGAAT TGAATTGAAA
4021 AGCTAGCTTA TCGATGATAA GCTGTCAAAG ATGAGAATTA ATTCCACGGA CTATAGACTA
4081 TACTAGATAC TCCGTCTACT GTACGATACA CTTCCGCTCA GGTCCTTGTC CTTTAACGAG
4141 GCCTTACCAC TCTTTTGTTA CTCTATTGAT CCAGCTCAGC AAAGGCAGTG TGATCTAAGA
4201 TTCTATCTTC GCGATGTAGT AAAACTAGCT AGACCGAGAA AGAGACTAGA AATGCAAAAG
4261 GCACTTCTAC AATGGCTGCC ATCATTATTA TCCGATGTGA CGCTGCAGCT TCTCAATGAT
4321 ATTCGAATAC GCTTTGAGGA GATACAGCCT AATATCCGAC AAACTGTTTT ACAGATTTAC
4381 GATCGTACTT GTTACCCATC ATTGAATTTT GAACATCCGA ACCTGGGAGT TTTCCCTGAA
4441 ACAGATAGTA TATTTGAACC TGTATAATAA TATATAGTCT AGCGCTTTAC GGAAGACAAT
4501 GTATGTATTT CGGTTCCTGG AGAAACTATT GCATCTATTG CATAGGTAAT CTTGCACGTC
4561 GCATCCCCGG TTCATTTTCT GCGTTTCCAT CTTGCACTTC AATAGCATAT CTTTGTTAAC
4621 GAAGCATCTG TGCTTCATTT TGTAGAACAA AAATGCAACG CGAGAGCGCT AATTTTTCAA
4681 ACAAAGAATC TGAGCTGCAT TTTTACAGAA CAGAAATGCA ACGCGAAAGC GCTATTTTAC
4741 CAACGAAGAA TCTGTGCTTC ATTTTTGTAA AACAAAAATG CAACGCGACG AGAGCGCTAA
4801 TTTTTCAAAC AAAGAATCTG AGCTGCATTT TTACAGAACA GAAATGCAAC GCGAGAGCGC
4861 TATTTTACCA ACAAAGAATC TATACTTCTT TTTTGTTCTA CAAAAATGCA TCCCGAGAGC
4921 GCTATTTTTC TAACAAAGCA TCTTAGATTA CTTTTTTTCT CCTTTGTGCG CTCTATAATG
4981 CAGTCTCTTG ATAACTTTTT GCACTGTAGG TCCGTTAAGG TTAGAAGAAG GCTACTTTGG
5041 TGTCTATTTT CTCTTCCATA AAAAAAGCCT GACTCCACTT CCCGCGTTTA CTGATTACTA
5101 GCGAAGCTGC GGGTGCATTT TTTCAAGATA AAGGCATCCC CGATTATATT CTATACCGAT
5161 GTGGATTGCG CATACTTTGT GAACAGAAAG TGATAGCGTT GATGATTCTT CATTGGTCAG
5221 AAAATTATGA ACGGTTTCTT CTATTTTGTC TCTATATACT ACGTATAGGA AATGTTTACA
5281 TTTTCGTATT GTTTTCGATT CACTCTATGA ATAGTTCTTA CTACAATTTT TTTGTCTAAA
5341 GAGTAATACT AGAGATAAAC ATAAAAAATG TAGAGGTCGA GTTTAGATGC AAGTTCAAGG
5401 AGCGAAAGGT GGATGGGTAG GTTATATAGG GATATAGCAC AGAGATATAT AGCAAAGAGA
5461 TACTTTTGAG CAATGTTTGT GGAAGCGGTA TTCGCAATGG GAAGCTCCAC CCCGGTTGAT
5521 AATCAGAAAA GCCCCAAAAA CAGGAAGATT GTATAAGCAA ATATTTAAAT TGTAAACGTT
5581 AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACGAATAG
5641 CCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA CCGAGATAGG GTTGAGTGTT
5701 GTTCCAGTTT CCAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA
5761 AAAAGGGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCTAATC AAGTTTTTTG
5821 GGGTCGAGGT GCCGTAAAGC AGTAAATCGG AAGGGTAAAC GGATGCCCCC ATTTAGAGCT
5881 TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGG
5941 GCTAGGGCGG TGGGAAGTGT AGGGGTCACG CTGGGCGTAA CCACCACACC CGCCGCGCTT
6001 AATGGGGCGC TACAGGGCGC GTGGGGATGA TCCACTAGT
//
DEFINITION pYES2/NT B
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..6039
/organism="pYES2/NT B"
/mol_type="other DNA"
promoter 1..451
/label="GAL1_promoter"
misc_feature 414..437
/label="GAL1_primer"
promoter 475..493
/label="T7_promoter"
misc_feature 542..560
/label="Xpress_fwd_primer"
misc_feature 543..575
/label="T7_leader"
misc_feature 579..602
/label="Xpress_EK"
terminator 792..1037
/label="CYC1_terminator"
misc_feature complement(801..819)
/label="CYC1_primer"
misc_feature complement(801..819)
/label="pYESTrp_rev_primer"
rep_origin complement(1265..1884)
/label="pBR322_origin"
CDS complement(2039..2899)
/label="ORF frame 3"
CDS complement(2995..3798)
/label="ORF frame 1"
gene complement(2998..3798)
/label="URA3"
/gene="URA3"
CDS 3287..3763
/label="ORF frame 2"
promoter complement(3800..4025)
/label="URA3_promoter"
rep_origin 4028..5499
/label="2micron_origin"
ORIGIN
1 ACGGATTAGA AGCCGCCGAG CGGGTGACAG CCCTCCGAAG GAAGACTCTC CTCCGTGCGT
61 CCTCGTCTTC ACCGGTCGCG TTCCTGAAAC GCAGATGTGC CTCGCGCCGC ACTGCTCCGA
121 ACAATAAAGA TTCTACAATA CTAGCTTTTA TGGTTATGAA GAGGAAAAAT TGGCAGTAAC
181 CTGGCCCCAC AAACCTTCAA ATGAACGAAT CAAATTAACA ACCATAGGAT GATAATGCGA
241 TTAGTTTTTT AGCCTTATTT CTGGGGTAAT TAATCAGCGA AGCGATGATT TTTGATCTAT
301 TAACAGATAT ATAAATGCAA AAACTGCATA ACCACTTTAA CTAATACTTT CAACATTTTC
361 GGTTTGTATT ACTTCTTATT CAAATGTAAT AAAAGTATCA ACAAAAAATT GTTAATATAC
421 CTCTATACTT TAACGTCAAG GAGAAAAAAC CCCGGATCGG ACTACTAGCA GCTGTAATAC
481 GACTCACTAT AGGGAATATT AAGCTTACCA TGGGGGGTTC TCATCATCAT CATCATCATG
541 GTATGGCTAG CATGACTGGT GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA
601 AGGTACCTAA GGATCCAGTG TGGTGGAATT CTGCAGATAT CCAGCACAGT GGCGGCCGCT
661 CGAGTCTAGA GGGCCCTTCG AAGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC
721 TACGCGTACC GGTCATCATC ACCATCACCA TTGAGTTTAA ACCCGCTGAT CCTAGAGGGC
781 CGCATCATGT AATTAGTTAT GTCACGCTTA CATTCACGCC CTCCCCCCAC ATCCGCTCTA
841 ACCGAAAAGG AAGGAGTTAG ACAACCTGAA GTCTAGGTCC CTATTTATTT TTTTATAGTT
901 ATGTTAGTAT TAAGAACGTT ATTTATATTT CAAATTTTTC TTTTTTTTCT GTACAGACGC
961 GTGTACGCAT GTAACATTAT ACTGAAAACC TTGCTTGAGA AGGTTTTGGG ACGCTCGAAG
1021 GCTTTAATTT GCAAGCTGCG GCCCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG
1081 GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC
1141 GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG
1201 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGCCCAGG AACCGTAAAA
1261 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC
1321 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC
1381 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG
1441 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT
1501 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC
1561 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC
1621 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG
1681 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG
1741 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA
1801 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG
1861 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT
1921 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA
1981 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT
2041 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG
2101 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GCGCTTACCA TCTGGCCCCA
2161 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC
2221 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT
2281 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG
2341 TTGTTGGCAT TGCTACAGGC ATCGTGGTGT CACTCTCGTC GTTTGGTATG GCTTCATTCA
2401 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG
2461 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA
2521 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG
2581 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT
2641 CTTGCCCGGC GTCAATACGG GATAATAGTG TATCACATAG CAGAACTTTA AAAGTGCTCA
2701 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA
2761 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG
2821 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC
2881 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATGGGT AATAACTGAT ATAATTAAAT
2941 TGAAGCTCTA ATTTGTGAGT TTAGTATACA TGCATTTACT TATAATACAG TTTTTTAGTT
3001 TTGCTGGCCG CATCTTCTCA AATATGCTTC CCAGCCTGCT TTTCTGTAAC GTTCACCCTC
3061 TACCTTAGCA TCCCTTCCCT TTGCAAATAG TCCTCTTCCA ACAATAATAA TGTCAGATCC
3121 TGTAGAGACC ACATCATCCA CGGTTCTATA CTGTTGACCC AATGCGTCTC CCTTGTCATC
3181 TAAACCCACA CCGGGTGTCA TAATCAACCA ATCGTAACCT TCATCTCTTC CACCCATGTC
3241 TCTTTGAGCA ATAAAGCCGA TAACAAAATC TTTGTCGCTC TTCGCAATGT CAACAGTACC
3301 CTTAGTATAT TCTCCAGTAG ATAGGGAGCC CTTGCATGAC AATTCTGCTA ACATCAAAAG
3361 GCCTCTAGGT TCCTTTGTTA CTTCTTCTGC CGCCTGCTTC AAACCGCTAA CAATACCTGG
3421 GCCCACCACA CCGTGTGCAT TCGTAATGTC TGCCCATTCT GCTATTCTGT ATACACCCGC
3481 AGAGTACTGC AATTTGACTG TATTACCAAT GTCAGCAAAT TTTCTGTCTT CGAAGAGTAA
3541 AAAATTGTAC TTGGCGGATA ATGCCTTTAG CGGCTTAACT GTGCCCTCCA TGGAAAAATC
3601 AGTCAAGATA TCCACATGTG TTTTTAGTAA ACAAATTTTG GGACCTAATG CTTCAACTAA
3661 CTCCAGTAAT TCCTTGGTGG TACGAACATC CAATGAAGCA CACAAGTTTG TTTGCTTTTC
3721 GTGCATGATA TTAAATAGCT TGGCAGCAAC AGGACTAGGA TGAGTAGCAG CACGTTCCTT
3781 ATATGTAGCT TTCGACATGA TTTATCTTCG TTTCCTGCAG GTTTTTGTTC TGTGCAGTTG
3841 GGTTAAGAAT ACTGGGCAAT TTCATGTTTC TTCAACACTA CATATGCGTA TATATACCAA
3901 TCTAAGTCTG TGCTCCTTCC TTCGTTCTTC CTTCTGTTCG GAGATTACCG AATCAAAAAA
3961 ATTTCAAAGA AACCGAAATC AAAAAAAAGA ATAAAAAAAA AATGATGAAT TGAATTGAAA
4021 AGCTAGCTTA TCGATGATAA GCTGTCAAAG ATGAGAATTA ATTCCACGGA CTATAGACTA
4081 TACTAGATAC TCCGTCTACT GTACGATACA CTTCCGCTCA GGTCCTTGTC CTTTAACGAG
4141 GCCTTACCAC TCTTTTGTTA CTCTATTGAT CCAGCTCAGC AAAGGCAGTG TGATCTAAGA
4201 TTCTATCTTC GCGATGTAGT AAAACTAGCT AGACCGAGAA AGAGACTAGA AATGCAAAAG
4261 GCACTTCTAC AATGGCTGCC ATCATTATTA TCCGATGTGA CGCTGCAGCT TCTCAATGAT
4321 ATTCGAATAC GCTTTGAGGA GATACAGCCT AATATCCGAC AAACTGTTTT ACAGATTTAC
4381 GATCGTACTT GTTACCCATC ATTGAATTTT GAACATCCGA ACCTGGGAGT TTTCCCTGAA
4441 ACAGATAGTA TATTTGAACC TGTATAATAA TATATAGTCT AGCGCTTTAC GGAAGACAAT
4501 GTATGTATTT CGGTTCCTGG AGAAACTATT GCATCTATTG CATAGGTAAT CTTGCACGTC
4561 GCATCCCCGG TTCATTTTCT GCGTTTCCAT CTTGCACTTC AATAGCATAT CTTTGTTAAC
4621 GAAGCATCTG TGCTTCATTT TGTAGAACAA AAATGCAACG CGAGAGCGCT AATTTTTCAA
4681 ACAAAGAATC TGAGCTGCAT TTTTACAGAA CAGAAATGCA ACGCGAAAGC GCTATTTTAC
4741 CAACGAAGAA TCTGTGCTTC ATTTTTGTAA AACAAAAATG CAACGCGACG AGAGCGCTAA
4801 TTTTTCAAAC AAAGAATCTG AGCTGCATTT TTACAGAACA GAAATGCAAC GCGAGAGCGC
4861 TATTTTACCA ACAAAGAATC TATACTTCTT TTTTGTTCTA CAAAAATGCA TCCCGAGAGC
4921 GCTATTTTTC TAACAAAGCA TCTTAGATTA CTTTTTTTCT CCTTTGTGCG CTCTATAATG
4981 CAGTCTCTTG ATAACTTTTT GCACTGTAGG TCCGTTAAGG TTAGAAGAAG GCTACTTTGG
5041 TGTCTATTTT CTCTTCCATA AAAAAAGCCT GACTCCACTT CCCGCGTTTA CTGATTACTA
5101 GCGAAGCTGC GGGTGCATTT TTTCAAGATA AAGGCATCCC CGATTATATT CTATACCGAT
5161 GTGGATTGCG CATACTTTGT GAACAGAAAG TGATAGCGTT GATGATTCTT CATTGGTCAG
5221 AAAATTATGA ACGGTTTCTT CTATTTTGTC TCTATATACT ACGTATAGGA AATGTTTACA
5281 TTTTCGTATT GTTTTCGATT CACTCTATGA ATAGTTCTTA CTACAATTTT TTTGTCTAAA
5341 GAGTAATACT AGAGATAAAC ATAAAAAATG TAGAGGTCGA GTTTAGATGC AAGTTCAAGG
5401 AGCGAAAGGT GGATGGGTAG GTTATATAGG GATATAGCAC AGAGATATAT AGCAAAGAGA
5461 TACTTTTGAG CAATGTTTGT GGAAGCGGTA TTCGCAATGG GAAGCTCCAC CCCGGTTGAT
5521 AATCAGAAAA GCCCCAAAAA CAGGAAGATT GTATAAGCAA ATATTTAAAT TGTAAACGTT
5581 AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACGAATAG
5641 CCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA CCGAGATAGG GTTGAGTGTT
5701 GTTCCAGTTT CCAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA
5761 AAAAGGGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT CACCCTAATC AAGTTTTTTG
5821 GGGTCGAGGT GCCGTAAAGC AGTAAATCGG AAGGGTAAAC GGATGCCCCC ATTTAGAGCT
5881 TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGG
5941 GCTAGGGCGG TGGGAAGTGT AGGGGTCACG CTGGGCGTAA CCACCACACC CGCCGCGCTT
6001 AATGGGGCGC TACAGGGCGC GTGGGGATGA TCCACTAGT
//
載體質(zhì)粒圖譜pdf版和相關(guān)資料下載
載體應(yīng)用舉例
pYIP5
pDR195
pPICZα A
pHIC-PI
pRS426gal
pYEPlac112
pYX212
pFLD/CAT
pPICZα B
pESC-Leu
pYES2/CT
pYES2-EGFP
pMET C
pPink-LC
pGAPZα C
pGAPZ B
pPIC9K
pPIC9
SUMO protease
pGADT7
pYES2
pPIC ZαD
Ycplac33
p416GFD
pYRP7
pRS41H
pRS316
pESC-His
pYES2-NTC
pMETαC
pMET B
pPink-HC
pGAPZα B
pGAPZ A
pAO815
pPIC9k-His
pPICZ A
BFD15
pPIC ZαGB
pYEPlac195
pADH2
pYCP211
pFA6a-FGP(S65T)-kanMX6
pESC-URA
pYES6/CT
pYES2-NTA
pMETαB
pMET A
pHIL-D2
pGAPZα A
pPICZα C
pPIC3.5K
pAUR123
pPICZ C
LA503
pPIC ZαFC
Ycp22lac-EGFP
pYIP211
pYES2-kan
pRS426
pESC-TRP
pYES3/CT
pYES2-NTB
pMETαA
pPinkα-HC
pHIL-S1
pGAPZ C
pPICZ B
pPIC3.5
pSH47
pSH63
pSH65
pGBKT7
pHis2
pHisSi-1
pDest22
pGBKT7-53
pMyr
pGADT7-T
p53blue
pSos MAFB
pSos
p53his
pCL1
pGBKT7-Lam
pACT2 AD
pGAG424
pDest32
pBridge
pYD1
pDisplay
AH109
PichiaPink Strain3
SMD1168H
X-33
INVSC1
PichiaPink Strain2
SMD1163
KM71
SMD1168
PichiaPink Strain1
KM71H
PichiaPink Strain4
EBY100
GS115
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