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首頁(yè) ? pET-17b圖譜序列抗性價(jià)格Biovector質(zhì)粒庫(kù)

pET-17b圖譜序列抗性價(jià)格Biovector質(zhì)粒庫(kù)

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pET17b載體基本信息

出品公司:別名:質(zhì)粒類型:表達(dá)水平:克隆方法:載體大小:5' 測(cè)序引物:5' 測(cè)序引物序列:  載體標(biāo)簽:載體抗性:備注:產(chǎn)品目錄號(hào):穩(wěn)定性:組成型:病毒/非病毒:
EMD Biosciences(Novagen)
pET17b, pet 17b
大腸桿菌蛋白表達(dá)
高
多克隆位點(diǎn)，限制性內(nèi)切酶
3306bp
T7
5'-TAATACGACTCACTATAGGG-3'
N-T7
Ampicillin
Hosts: E.coli. Related vectors: pBR322.
69663-3
瞬時(shí)表達(dá) Transient
組成型 Constitutive
非病毒

pET17b載體質(zhì)粒圖譜和多克隆位點(diǎn)信息

pet17b載體圖譜


pet17b載體多克隆位點(diǎn)

Feature Name
Start
End
 
T7_terminator1313
T7_Terminal_primer7189
T7_leader269237
Xpress_fwd_primer270252
T7_transl_en_RBS294278
T7_promoter349331
ROP8581049
pGEX_3_primer10651043
Ampicillin30982238
AmpR_promoter31683140
ORF
Start
End
 
ORF frame 230982238
Enzyme Name
Cut
XhoI141
NotI147
EcoRV166
EcoRI174
SpeI199
BamHI205
SacI215
KpnI221
HindIII223
NheI261
NdeI268
XbaI306
BglII364
MscI389
PvuII1009
AatII3233

pET17b載體簡(jiǎn)介

The pET-17b vector (Cat. No. 69663-3) carries an N-terminal 11aa T7?Tag? sequence followed by a region of useful cloning sites. Included in the multiple cloning region are dual BstX I sites, which allow efficient cloning using an asymmetric linker. Unique sites (except for the two BstX I sites) are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circluar map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below.

pET17b載體序列

LOCUS       pET-17b    3306 bp     DNA   circular  SYN
DEFINITION  pET-17b
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="pET-17b"
                     /mol_type="other DNA"
     terminator      complement(3..131)
                     /label="T7_terminator"
     misc_feature    71..89
                     /label="T7_Terminal_primer"
     misc_feature    complement(237..269)
                     /label="T7_leader"
     misc_feature    complement(252..270)
                     /label="Xpress_fwd_primer"
     misc_feature    complement(278..294)
                     /label="T7_transl_en_RBS"
     promoter        complement(331..349)
                     /label="T7_promoter"
     misc_feature    858..1049
                     /label="ROP"
     misc_feature    complement(1043..1065)
                     /label="pGEX_3_primer"
     gene            complement(2238..3098)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(2238..3098)
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(3140..3168)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
   

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