LOCUS pET-26b(+) 5360 bp DNA circular SYN
DEFINITION pET-26b(+)
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
source 1..5360
/organism="pET-26b(+)"
/mol_type="other DNA"
terminator complement(1..129)
/label="T7_terminator"
misc_feature 69..87
/label="T7_Terminal_primer"
misc_feature complement(298..314)
/label="T7_transl_en_RBS"
misc_feature complement(332..359)
/label="lacO"
promoter complement(359..377)
/label="T7_promoter"
gene 409..672
/label="tet (300 - 563)"
/gene="tet (300 - 563)"
misc_feature complement(461..480)
/label="pBRrevBam_primer"
CDS 514..1014
/label="ORF frame 1"
misc_feature 755..1846
/label="lacI"
CDS 887..1846
/label="ORF frame 2"
gene 1905..2180
/label="tet (576 - 851)"
/gene="tet (576 - 851)"
misc_feature 2655..2846
/label="ROP"
misc_feature complement(2840..2862)
/label="pGEX_3_primer"
rep_origin complement(3261..3880)
/label="pBR322_origin"
gene 3986..4801
/label="KanR2"
/gene="KanR2"
CDS 3986..4801
/label="ORF frame 2"
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
181 cgacggagct cgaattcgga tccgaattaa ttccgatatc catggccatc gccggctggg
241 cagcgaggag cagcagacca gcagcagcgg tcggcagcag gtatttcata tgtatatctc
301 cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc acaattcccc
361 tatagtgagt cgtattaatt tcgcgggatc gagatctcga tcctctacgc cggacgcatc
421 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
481 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
541 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
601 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
661 cataagggag agcgtcgaga tcccggacac catcgaatgg cgcaaaacct ttcgcggtat
721 ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt
781 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca
841 ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa
901 ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt
961 tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg
1021 cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc
1081 ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta
1141 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt
1201 atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg
1261 tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc
1321 gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac
1381 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt
1441 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa
1501 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc
1561 ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt
1621 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca
1681 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag
1741 aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
1801 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
1861 atgtaagtta gctcactcat taggcaccgg gatctcgacc gatgcccttg agagccttca
1921 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
1981 tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg
2041 aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct
2101 tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc
2161 aggccattat cgccggcatg gcggccccac gggtgcgcat gatcgtgctc ctgtcgttga
2221 ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg
2281 agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg
2341 tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta
2401 tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat
2461 taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg
2521 ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta
2581 tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt
2641 acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca
2701 gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag
2761 acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt
2821 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
2881 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
2941 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
3001 tgcggcatca gagcagattg tactgagagt gcaccatata tgcggtgtga aataccgcac
3061 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
3121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
3181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
3241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
3301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
3361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
3421 ac