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首頁 ? pET-42b(+)圖譜序列抗性價(jià)格Biovector質(zhì)粒庫

pET-42b(+)圖譜序列抗性價(jià)格Biovector質(zhì)粒庫

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pet42b載體基本信息

出品公司:別名:質(zhì)粒類型:表達(dá)水平:克隆方法:載體大小:5' 測序引物:5' 測序引物序列:  載體標(biāo)簽:載體抗性:備注:產(chǎn)品目錄號:穩(wěn)定性:組成型:病毒/非病毒:
EMD Biosciences(Novagen)
pET42b, pet 42b, pET-42b(+)
大腸桿菌蛋白表達(dá)
高
多克隆位點(diǎn)，限制性內(nèi)切酶
5929bp
T7
5'-TAATACGACTCACTATAGGG-3'
N-GST, N-His, C-His
Kanamycin
含有GST融合蛋白;N端Thrombin蛋白酶切位點(diǎn); 含有N端Factor Xa蛋白酶切位點(diǎn) ; pET 42 a,b,c 的差異僅在多克隆位點(diǎn)區(qū)域。
70562-3
瞬時(shí)表達(dá) Transient
組成型 Constitutive
非病毒

pet42b載體質(zhì)粒圖譜和多克隆位點(diǎn)信息

pet42b載體圖譜
pet42b多克隆位點(diǎn)

Feature Name
Start
End
 
T7_terminator1201
T7_Terminal_primer6987
S15350306
6xHIS410393
GST (variant)1091441
T7_transl_en_RBS11161100
lacO11611134
T7_promoter11791161
tet (300 - 563)12151478
pBRrevBam_primer12861267
lacI15612652
ROP32243415
pGEX_3_primer34313409
pBR322_origin44493830
KanR245555370
ORF
Start
End
 
ORF frame 31091147
ORF frame 310921820
ORF frame 116932652
ORF frame 145555370
Enzyme Name
Cut
XhoI174
NotI182
EagI182
HindIII189
SalI195
SacI206
PstI214
AscI216
StuI226
EcoRI235
BamHI241
EcoRV251
NcoI254
AgeI288
KpnI295
BglII298
SacII389
SpeI420
MscI883
NdeI1090
XbaI1128
ApaI2131
HpaI2426
NruI4643
SmaI4860
XmaI4858

pet42b載體簡介

The pET-42 series is designed for cloning and high-level expression of peptide sequencesfused with the 220 aa GST•Tag™ protein. Unique sites are shown on the circle map. Note that thesequence is numbered by the pBR322 convention, so the T7 expression region is reversed on thecircle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymeraseis shown below. The f1 origin is oriented so that infection with helper phage will produce virionscontaining single stranded DNA that corresponds to the coding strand. Therefore, single strandedsequencing should be performed using the T7 terminator primer (cat. no. 69337-3). Vector encodedsequence can be completely removed when cloning into the PshAI site (as shown below) and thencleaving the GST fusion protein with Factor Xa.

pet42b載體序列

LOCUS pET-42b(+) 5929 bp DNA circular SYN
DEFINITION pET-42b(+), pet42b
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|Biofeng.com|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
source 1..5929
/organism="pET-42 b(+)"
/mol_type="other DNA"
terminator complement(1..120)
/label="T7_terminator"
misc_feature 69..87
/label="T7_Terminal_primer"
CDS complement(147..1091)
/label="ORF frame 3"
misc_feature complement(306..350)
/label="S15"
misc_feature complement(393..410)
/label="6xHIS"
gene complement(441..1091)
/label="GST (variant)"
/gene="GST (variant)"
CDS 1092..1820
/label="ORF frame 3"
misc_feature complement(1100..1116)
/label="T7_transl_en_RBS"
misc_feature complement(1134..1161)
/label="lacO"
promoter complement(1161..1179)
/label="T7_promoter"
gene 1215..1478
/label="tet (300 - 563)"
/gene="tet (300 - 563)"
misc_feature complement(1267..1286)
/label="pBRrevBam_primer"
misc_feature 1561..2652
/label="lacI"
CDS 1693..2652
/label="ORF frame 1"
misc_feature 3224..3415
/label="ROP"
misc_feature complement(3409..3431)
/label="pGEX_3_primer"
rep_origin complement(3830..4449)
/label="pBR322_origin"
gene 4555..5370
/label="KanR2"
/gene="KanR2"
CDS 4555..5370
/label="ORF frame 1"
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gac

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