LOCUS       pET-49b(+)              5926 bp    DNA     circular     11-SEP-2008
DEFINITION  pET-49b(+).
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
FEATURES             Location/Qualifiers
     rep_origin      complement(5467..5914)
                     /vntifkey="33"
                     /label=f1\ori
     misc_RNA        complement(3843..4398)
                     /vntifkey="53"
                     /label=RNA\II
     rep_origin      complement(3843..3843)
                     /vntifkey="33"
                     /label=ColE1,\pBR\ori
     misc_RNA        4283..4390
                     /vntifkey="53"
                     /label=RNA\I
     CDS             3221..3412
                     /vntifkey="4"
                     /label=ROP\gene
     CDS             1567..2649
                     /vntifkey="4"
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(1160..1176)
                     /vntifkey="30"
                     /label=T7\promoter
     protein_bind    complement(1135..1155)
                     /vntifkey="31"
                     /label=lacO
     CDS             complement(429..1088)
                     /vntifkey="4"
                     /label=GST
     protein_bind    complement(342..359)
                     /vntifkey="31"
                     /label=His-Tag
     terminator      complement(26..73)
                     /vntifkey="43"
                     /label=T7\terminator
     CDS             4552..5367
                     /vntifkey="4"
                     /label=Kan
     misc_feature    complement(213..230)
                     /vntifkey="21"
                     /label=Thrombin\site
     protein_bind    complement(168..212)
                     /vntifkey="31"
                     /label=S-Tag
     protein_bind    complement(310..332)
                     /vntifkey="31"
                     /label=3c
     primer          complement(300..336)
                     /vntifkey="27"
                     /label=S-SacII/Bam\+SanDI\-dcm
     primer          complement(374..421)
                     /vntifkey="27"
                     /label=S-Tag\Spacer-Spe\I
BASE COUNT     1485 a      1486 c      1520 g      1435 t
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgtttagcag cctaggttaa ttaagcctcg agagcagcag aagtagagct gtccatgtgc
      181 tggcgttcga atttagcagc agcggtttct ttactaccgc gtggcaccag agcgagctct
      241 gcggccgcaa gcttgtcgac ggacgtcggg cgcgccaagg cctgtacaga attcggatcc
      301 tggtacccgg gtccctgaaa gaggacttca agagccgcgg agtgatggtg atggtgatga
      361 ccagaaccac tagatggagt aggagtagga ggattgttat tagaaccgcc accaccacta
      421 gttgaaccat ccgattttgg aggatggtcg ccaccaccaa acgtggcttg ccagccctgc
      481 aaaggccatg ctatatactt gctggatttc aagtacttat caatttgtgg gatagcttca
      541 atacgttttt taaaacaaac taattttggg aacgcatcca ggcacattgg gtccatgtat
      601 aaaacaacat caagagcgtc atacaacatg aagtcaggat gggttacatg atcaccattt
      661 aaatatgttt tatgacataa acgatcttcg aacattttca gcatttcagg tagcttgcta
      721 agaaaatcaa ctttgagagt ttcaaagtct ttactatatg caattctcga aacaccgtat
      781 ctaatatcca aaaccgctcc ttcaagcatt gaaatctctg cacgctcttt tggacaacca
      841 cccaacatgt tgtgcttgtc agctatataa cgtatgatgg ccatagactg tgttaattta
      901 acatcaccat caatataata aggaagattg ggaaactcca aacccaattc aaactttttg
      961 tttcgccatt tatcaccttc atcgcgctca tacaaatgct cttcatattt ttcttcaaga
     1021 tattccaaaa gaagtcgagt gggttgcaca aggcccttaa ttttccaata acctagtata
     1081 ggggacatat gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt
     1141 tatccgctca caattcccct atagtgagtc gtattaattt cgcgggatcg agatcgatct
     1201 cgatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg
     1261 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga
     1321 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca
     1381 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg
     1441 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg agatcccgga caccatcgaa
     1501 tggcgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg
     1561 gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag
     1621 accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg
     1681 gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc
     1741 aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa
     1801 attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg
     1861 gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc
     1921 gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct
     1981 gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt
     2041 attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt
     2101 caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg
     2161 gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc
     2221 gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt
     2281 cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc
     2341 gagtccgggc tgcgcgttgg tgcggacatc tcggtagtgg gatacgacga taccgaagac
     2401 agctcatgtt atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa
     2461 accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg
     2521 ttgcccgtct cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct
     2581 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     2641 gggcagtgag cgcaacgcaa ttaatgtaag ttagctcact cattaggcac cgggatctcg
     2701 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
     2761 tagcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg
     2821 ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc
     2881 tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac
     2941 gcggaagtca gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct