- BioVector NTCC典型培養物保藏中心
- 聯系人：Dr.Xu, Biovector NTCC Inc.
電話：400-800-2947 工作QQ:1843439339 (微信同號)
郵件：Biovector@163.com
手機：18901268599
地址：北京
- 已注冊
Dr Xu,Senior Scientist
電郵Biovector@163.com
電話1890I268599
訂購合同下載請點擊：Biovector質粒載體菌株細胞基因訂購合同-Biovector Science Lab
^_^尋找您所需質粒載體菌株基因細胞株等資源的竅門：在Google，Baidu等搜索引擎中搜索“Biovector您所需的資源名稱”結果即刻閃現，馬上試試吧^_^
點擊瀏覽器菜單“編輯”-->"查找"或者直接按“Ctrl+F”查找您所需資源
由于頁面字數限制，尚有很多質粒載體菌株基因片段名稱未列出，如查找不到您所需資源，請發郵件或電話查詢。
Biovector,Inc質粒載體菌株細胞株基因保藏中心列表網址http://www.Biovector.net
標簽：以下資源由Biovector Inc. 質粒載體菌株細胞株基因保藏中心獨家供應并發布，侵權必究。Biovector,Inc質粒載體菌株細胞株基因保藏中心列表網址http://biovector.blog.163.com質粒 載體 索取 報價 價格 優惠 售價 出售 圖譜 序列 酶切位點 轉化 構建 求購 購買 訂購 sequence map plasmid vector gene strain order ship 抗性 resistance restriction site
產品分類
| NEB（New England Biolabs） |
| pMXB10 |
| 原核細胞表達載體 |
| -- |
| T7 |
| 多克隆位點，限制性內切酶 |
| 7816bp |
| -- |
| -- |
| -- |
| 氨芐青霉素(Ampicillin) |
| -- |
| -- |
| E6903 |
| -- |
| -- |
| -- |
載體質粒圖譜和多克隆位點信息
載體簡介
E. coli malE gene, encoding the maltose binding protein (MBP, 42 kDa) (3), fused in-frame to
the coding region of the Mxe gyrase A intein-chitin binding domain (28 kDa) from pTXB1 (2,4,5).
pMXB10 can be used to test plasmid transformation, cell culture, induction and purification
procedures. After induction with 0.3 mM IPTG at 30°C for 3 hours (or 15°C for 12-16 hours),
100 ml of cells should yield 2-3 mg of a 70 kDa fusion protein. After chitin column purification
and cleavage, approximately 1.5-2.0 mg of MBP is usually obtained. This double stranded vector
is 7813 bp in length.
載體序列
DEFINITION Control vector pMXB10, complete sequence.
ACCESSION
VERSION
KEYWORDS .
SOURCE Control vector pMXB10
ORGANISM Control vector pMXB10
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7816)
AUTHORS Telenti,A., Southworth,M., Alcaide,F., Daugelat,S., Jacobs,W.R. Jr.
and Perler,F.B.
TITLE The Mycobacterium xenopi GyrA protein splicing element:
characterization of a minimal intein
JOURNAL J Bacteriol 179, 6378-6382 (1997)
PUBMED 9335286
REFERENCE 2 (bases 1 to 7816)
AUTHORS Evans,T.C. Jr., Benner,J. and Xu,M.-Q.
TITLE Semisynthesis of cytotoxic proteins using a modified protein
splicing element
JOURNAL Protein Sci 7, 2256-2264 (1998)
PUBMED 9827992
REFERENCE 3 (bases 1 to 7816)
AUTHORS Southworth,M.W., Amaya,K., Evans,T.C., Xu,M.-Q. and Perler,F.B.
TITLE Purification of proteins fused to either the amino or carboxy
terminus of the Mycobacterium xenopi Gyrase A intein
JOURNAL BioTechniques 27, 110-120 (1999)
PUBMED 10407673
REFERENCE 4 (bases 1 to 7816)
AUTHORS Perler,F.B.
TITLE InBase, the intein database
JOURNAL Nucleic Acids Res 30 (1), 383-384 (2002)
PUBMED 11752343
REFERENCE 5 (bases 1 to 7816)
AUTHORS Ghosh,I. and Xu,M.Q.
TITLE Direct Submission
JOURNAL Submitted (23-OCT-2007) Research Department, New England Biolabs,
240 County Road, Ipswich, MA 01938, USA
FEATURES Location/Qualifiers
source 1..7816
/organism="Control vector pMXB10"
/mol_type="other DNA"
gene 140..1000
/gene="bla"
CDS 140..1000
/gene="bla"
/note="ampR (confers resistance to ampicillin)"
/codon_start=1
/product="beta-lactamase"
sig_peptide 140..208
/gene="bla"
/note="required for secretion to the periplasm; cleaved
off to form the mature beta-lactamase protein"
rep_origin 1042..1555
/note="M13 origin of replication (+ -)"
rep_origin 1666..2254
/note="pMB1 origin of replication (clockwise) (RNAII -35
to RNA/DNA switch point)"
gene complement(2623..2814)
/gene="rop"
CDS complement(2623..2814)
/gene="rop"
/exception="alternative start codon"
/codon_start=1
/transl_table=11
/product="ROP protein"
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
ADELYRSCLARFGDDGENL"
您正在向 biovector.net 發送關于產品 pMXB10圖譜序列抗性價格Biovector質粒庫 的詢問
- 公告/新聞
訂單付款
Order & Payment
1.支付寶掃碼支付
2. 信用卡/貝寶支付 Pay by PayPal / Credit Card / Apple Pay
微信碼：
名片碼:
關于我們 下單訂購 加入我們 Copyright ? 1901-2024 京ICP備13016347號-3BioVector質粒載體菌種細胞蛋白抗體基因保藏中心-NTCC典型培養物保藏中心 -All Rights Reserved.
免費訂購電話: 400-800-2947
北京實驗室: 189-01268599
訂單及咨詢郵箱：Biovector@163.com
工作QQ/微信：1843439339
經銷商聯系：189-01268599