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載體基本信息
出品公司:
| Invitrogen |
載體名稱:
| pcDNA3.1/Hygro(+) |
質粒類型:
| 哺乳動物(mammal;mammalian)細胞表達載體 |
啟動子:
| CMV |
表達水平:
| 高 |
克隆方法:
| 多克隆位點，限制性內切酶 |
載體大小:
| 5597 bp |
5' 測序引物:
| pcDNA3.1-F:CTAGAGAACCCACTGCTTAC |
3' 測序引物:
| pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG |
載體標簽:
| C-His, C-Myc |
載體抗性:
| Ampicillin 氨芐 |
篩選標記:
| Hygromycin 潮霉素 |
備注:
Differs from other pcDNA3.1 in drug resistance; +/- refers to orientation of f1 ori. |
產品目錄號:
| V870-20 |
穩定性:
| 瞬表達 Transient |
組成型:
| 組成型 Constitutive |
病毒/非病毒:
| 非病毒 |
載體質粒圖譜和多克隆位點信息
載體簡介
pcDNA3.1/Hygro (+) and pcDNA3.1/Hygro (–) are 5.6 kb vectors derived from pcDNA3.1 and are designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. These vectors contain the following elements:
?Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells
?Multiple cloning sites in the forward (+) and reverse (–) orientations to facilitate cloning
?Hygromycin resistance gene for selection of stable cell lines
?Episomal replication in cells lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g., COS–1, COS–7)
A control plasmid, pcDNA3.1/Hygro/lacZ, is included for use as a positive control for transfection and expression in the cell line of choice.
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