BL21 star (DE3) pLysS菌株感受態(tài)細胞抗性價格報價Biovector菌株

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sequence map plasmid vector gene strain order ship抗性resistance restriction site

Description

One Shot® BL21 Star™ (DE3) pLysS E. coli are chemically competent cells designed for applications that require high-level expression of non-toxic recombinant proteins from high copy number, T7 promoter-based expression systems (e.g., pRSET T7 vectors). One Shot® BL21 Star™ (DE3) pLysS Chemically Competent cells have a transformation efficiency of >1 x 108 cfu/ µg plasmid DNA.
• High mRNA stability results in increased protein yield
• Ideal for use with high copy number, T7 promoter-based plasmids
• Low background expression in uninduced cells

Increased mRNA Stability Improves Protein Yield
One Shot® BL21 Star™ (DE3) pLysS E. coli offers enhanced mRNA stability so that abundant mRNA is available for protein expression. This enhanced stability is due to a mutation in the RNaseE gene (rne131), which is involved in mRNA degradation.

High Expression of Non-Toxic Recombinant Proteins
One Shot® BL21 Star™ (DE3) pLysS cells are ideal for high-level expression of non-toxic but potentially growth-inhibiting recombinant proteins from high copy number, T7 promoter-based expression systems. The pLysS CamR plasmid carried by the BL21 Star™ (DE3) pLysS strain expresses T7 lysozyme, a T7 RNA polymerase inhibitor that prevents leaky expression in uninduced cells. The p15a origin of pLysS makes this plasmid compatible with pUC or pBR322-derived plasmids. This strain also contains the DE3 lysogen that carries the T7 RNA polymerase gene under control of the lacUV5 promoter, and IPTG is required to induce expression of the T7 RNA polymerase. One Shot® BL21 Star™ (DE3) pLysS cells do not contain the lon protease and are also deficient in the outer membrane protease, OmpT. The absence of these proteases reduces degradation of heterologous proteins.

Note: The BL21 Star™ strains have higher basal expression of heterologous genes than BL21 strains, due to the increased stability of mRNA. Therefore, these strains may not be useful for expression of toxic genes. However, the BL21 Star™ (DE3) pLysS strains offer lower expression levels than the BL21 Star™ strains.

Genotype:
F-ompT hsdSB (rB-, mB-) galdcmrne131 (DE3) pLysS (CamR)

BL21(DE3)Star pLySs感受態(tài)細胞基本信息

出品公司:感受態(tài)細胞名稱:菌株類型:產(chǎn)品目錄號:培養(yǎng)基:生長條件:基因型:抗性:應用:菌株特點:

Invitrogen

BL21 Star(DE3)pLySs, BL21(DE3)Star pLySs

E.coli

C6020-03

LB培養(yǎng)基

37 ℃, 有氧

氯霉素

蛋白表達，IPTG誘導表達

來源于BL21(DE3)菌株。

含有rne131基因突變體，rne131突變基因能夠增強菌株細胞內(nèi)mRNA的穩(wěn)定性，從而有效提高蛋白表達能力。

主要適用于T7啟動子載體的高水平蛋白表達。

含有pLSs質(zhì)粒，pLySs質(zhì)粒能夠產(chǎn)生T7溶菌酶，可以有效降低目的基因的基礎(chǔ)表達水平。pLySs質(zhì)粒使得該菌株具有氯霉素抗性。pLySs質(zhì)粒的起始復制位點是p15 Origin, 這使得該質(zhì)粒能夠和pUC-及pBR322-衍生的質(zhì)粒互相共存。

目的基因在菌株中的基礎(chǔ)表達水平，一般是BL21 Star(DE3)要高于BL21 Star(DE3)pLySs，但是誘導后的總共的蛋白表達水平仍然是BL21 Star(DE3)要高于BL21 Star(DE3)pLySs。總得來說，目的基因的表達水平在BL21 Star(DE3)要高于在BL21 Star(DE3)pLySs中。

主要用來表達有毒蛋白或者對蛋白生長具有抑制作用的基因蛋白。

建議選擇該菌株進行蛋白表達的條件如下：1. 你在使用高拷貝的T7啟動子載體進行蛋白表達。2.使用BL21 Star(DE3)進行蛋白表達時，你觀察到明顯的對細菌生長的抑制作用。3.你在表達一個已知的毒性蛋白。