nce restriction site

Description

E. coli BL21 Star (DE3) is designed for applications that require high-level expression of non-toxic recombinant proteins from low copy number, T7 promoter-based expression systems (e.g., pET vectors).

• Contain a genotype that promotes high mRNA stability and protein yield
• Optimized for use with low copy number, T7 promoter-based plasmids
• Provided in a single-tube, single-use format—allows all transformation steps to take place in the same tube, thereby helping to save time and prevent contamination

Enhanced expression of nontoxic recombinant proteins
BL21 Star (DE3) cells contain the DE3 lysogen that carries the gene for T7 RNA polymerase under control of the lacUV5 promoter. IPTG is required to induce expression. This strainalso offers enhanced mRNA stability due to a mutation in the RNaseE gene (rne131) that reduces levels of endogenous RNases and mRNA degradation, thereby increasing the stability of mRNA transcripts and increasing protein yield. Protein expression is further enhanced by the absence of thelon and outer membrane (OmpT) proteases, which reduces degradation of heterologous proteins.

Note: The BL21 Star strains have higher basal expression of heterologous genes than BL21 strains, due to the increased stability of mRNA. Therefore, these strains may not be useful for expression of toxic genes.

Genotype:F^-ompT hsdS_B (r_B^-, m_B^-) galdcmrne131 (DE3)

 

BL21(DE3)Star感受態細胞基本信息

出品公司:感受態 細胞名稱:菌株類型:產品目錄號:培養基:生長條件:基因型:抗性:應用:菌株特點:

Invitrogen

BL21 Star(DE3), BL21(DE3)Star

E.coli

C6010-03

LB培養基

37 ℃, 有氧

無

蛋白表達

來源于BL21(DE3)菌株。 含有rne131基因突變體，rne131突變基因能夠增強菌株細胞內mRNA的穩定性，從而有效提高蛋白表達能力。 主要適用于T7啟動子載體的高水平蛋白表達。

BL21(DE3)Star感受態細胞說明書pdf版和相關資料下載

BL21(DE3)Star感受態細胞應用舉例