pQE-TriSystem質(zhì)粒載體圖譜序列抗性說(shuō)明書價(jià)格Biovector公司
- 價(jià) 格：￥3920
- 貨 號(hào)：BiovectorpQE-TriSystem
- 產(chǎn) 地：北京
- BioVector NTCC典型培養(yǎng)物保藏中心
- 聯(lián)系人：Dr.Xu, Biovector NTCC Inc.
電話：400-800-2947 工作QQ:1843439339 (微信同號(hào))
郵件：Biovector@163.com
手機(jī)：18901268599
地址：北京
- 已注冊(cè)
載體基本信息
| 出品公司: | Qiagen |
|---|---|
| 載體名稱: | pQE-TriSystem |
| 質(zhì)粒類型: | 原核表達(dá)載體；昆蟲表達(dá)載體；哺乳細(xì)胞表達(dá)載體 |
| 高拷貝/低拷貝: | 高拷貝 |
| 啟動(dòng)子: | P10啟動(dòng)子 |
| 克隆方法: | 多克隆位點(diǎn)，限制性內(nèi)切酶 |
| 載體大小: | 5766 bp |
| 5' 測(cè)序引物及序列: | -- |
| 3' 測(cè)序引物及序列: | -- |
| 載體標(biāo)簽: | 8xHis |
| 載體抗性: | 氨芐青霉素 |
| 篩選標(biāo)記: | -- |
| 備注: | 高水平蛋白表達(dá) |
| 產(chǎn)品目錄號(hào): | -- |
| 穩(wěn)定性: | 瞬時(shí)表達(dá) |
| 組成型: | 非組成型 |
| 病毒/非病毒: | 非病毒 |
載體質(zhì)粒圖譜和多克隆位點(diǎn)信息
載體簡(jiǎn)介
載體序列
LOCUS pQE-TriSystem 5766 bp DNA circular SYN
DEFINITION pQE-TriSystem
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biovector.net/
COMMENT VNTAUTHORNAME|biovector.net|
FEATURES Location/Qualifiers
source 1..5766
/organism="pQE-TriSystem"
/mol_type="other DNA"
misc_feature 68..355
/label="CAG_enhancer"
CDS complement(456..983)
/label="ORF frame 2"
misc_feature 1012..1031
/label="pCAG_F_primer"
terminator 1743..1778
/label="T7_terminator"
misc_feature complement(1801..1818)
/label="Baculovirs_rev_primer"
misc_feature 1808..1885
/label="3_AcPH"
CDS complement(1915..3168)
/label="ORF frame 1"
misc_feature complement(1997..2795)
/label="r_ORF1629"
gene complement(4015..4875)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(4015..4875)
/label="ORF frame 1"
CDS complement(5103..5708)
/label="ORF frame 2"
ORIGIN
1 CTCTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT ATATGGAGTT
61 CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC
121 ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG
181 TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT
241 GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA
301 GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT
361 TACCATGCAT GGTCGAGGTG AGCCCCACGT TCTGCTTCAC TCTCCCCATC TCCCCCCCCT
421 CCCCACCCCC AATTTTGTAT TTATTTATTT TTTAATTATT TTGTGCAGCG ATGGGGGCGG
481 GGGGGGGGGG GGGGCGCGCG CCAGGCGGGG CGGGGCGGGG CGAGGGGCGG GGCGGGGCGA
541 GGCGGAGAGG TGCGGCGGCA GCCAATCAGA GCGGCGCGCT CCGAAAGTTT CCTTTTATGG
601 CGAGGCGGCG GCGGCGGCGG CCCTATAAAA AGCGAAGCGC GCGGCGGGCG GGAGTCGTGC
661 GCGCTGCCTT CGCCCCGTGC CCCGCTCCGC CGCCGCCTCG CGCCGCCCGC CCCGGCTCTG
721 ACTGACCGCG TTACTCCCAC AGGTGAGCGG GCGGGACGGC CCTTCTCCTT CGGGCTGTAA
781 TTAGCGCTTG GTTTAATGAC GGCTTGTTTC TTTTCTGTGG CTGCGTGAAA GCCTTGAGGG
841 GCTCCGGGAG GGCCCTTTGT GCGGGGGGAG CGGCTCGGGG CTGTCCGCGG GGGGACGGCT
901 GCCTTCGGGG GGGACGGGGC AGGGCGGGGT TCGGCTTCTG GCGTGTGACC GGCGGCTCTA
961 GAGCCTCTGC TAACCATGTT CATGCCTTCT TCTTTTTCCT ACAGCTCCTG GGCAACGTGC
1021 TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTGGA TCGGACCGGA AATCATAAAA
1081 AATTATTTGC TTTGTGAGCG GATAACAATT ATAATAGATT CAATTGAGGC CTCGACCACC
1141 GGGACCTTTA ATTCAACCCA ACACAATATA TTATAGTTAA ATAAGAATTA TTATCAAATC
1201 ATTTGTATAT TAATTAAAAT ACTATACTGT AAATTACATT TTATTTACAA TCAAAGGAGA
1261 TATACCATGG CGATATCCCG GGAGCTCGTG GATCCGAATT CTCAGATCTC GGCGCGCCTG
1321 CAGGTCGACG GTACCGGTTC GAAGCTTGCG GCCGCACAGC TGTATACACG TGCAAGCCAG
1381 CCAGAACTCG CCCCGGAAGA CCCCGAGGAT CTCGAGCACC ACCATCACCA TCACCATCAC
1441 TAAGTGATTA ACCTCAGGTG CAGGCTGCCT ATCAGAAGGT GGTGGCTGGT GTGGCCAATG
1501 CCCTGGCTCA CAAATACCAC TGAGATCGAT CTTTTTCCCT CTGCCAAAAA TTATGGGGAC
1561 ATCATGAAGC CCCTTGAGCA TCTGACTTCT GGCTAATAAA GGAAATTTAT TTTCATTGCA
1621 ATAGTGTGTT GGAATTTTTT GTGTCTCTCA CTCGGAAGGA CATATGGGAG GGCAAATCAT
1681 TTAAAACATC AGAATGAGTT TTTGGTTTAG AGTTTGGCAA CATATGCCCA TATGTAACTA
1741 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGCATGCGGA
1801 GGAAATTCTC CTTGAAGTTT CCCTGGTGTT CAAAGTAAAG GAGTTTGCAC CAGACGCACC
1861 TCTGTTCACT GGTCCGGCGT ATTAAAACAC GATACATTGT TATTAGTACA TTTATTAAGC
1921 GCTAGATTCT GTGCGTTGTT GATTTACAGA CAATTGTTGT ACGTATTTTA ATAATTCATT
1981 AAATTTATAA TCTTTAGGGT GGTATGTTAG AGCGAAAATC AAATGATTTT CAGCGTCTTT
2041 ATATCTGAAT TTAAATATTA AATCCTCAAT AGATTTGTAA AATAGGTTTC GATTAGTTTC
2101 AAACAAGGGT TGTTTTTCCG AACCGATGGC TGGACTATCT AATGGATTTT CGCTCAACGC
2161 CACAAAACTT GCCAAATCTT GTAGCAGCAA TCTAGCTTTG TCGATATTCG TTTGTGTTTT
2221 GTTTTGTAAT AAAGGTTCGA CGTCGTTCAA AATATTATGC GCTTTTGTAT TTCTTTCATC
2281 ACTGTCGTTA GTGTACAATT GACTCGACGT AAACACGTTA AATAGAGCTT GGACATATTT
2341 AACATCGGGC GTGTTAGCTT TATTAGGCCG ATTATCGTCG TCGTCCCAAC CCTCGTCGTT
2401 AGAAGTTGCT TCCGAAGACG ATTTTGCCAT AGCCACACGA CGCCTATTAA TTGTGTCGGC
2461 TAACACGTCC GCGATCAAAT TTGTAGTTGA GCTTTTTGGA ATTATTTCTG ATTGCGGGCG
2521 TTTTTGGGCG GGTTTCAATC TAACTGTGCC CGATTTTAAT TCAGACAACA CGTTAGAAAG
2581 CGATGGTGCA GGCGGTGGTA ACATTTCAGA CGGCAAATCT ACTAATGGCG GCGGTGGTGG
2641 AGCTGATGAT AAATCTACCA TCGGTGGAGG CGCAGGCGGG GCTGGCGGCG GAGGCGGAGG
2701 CGGAGGTGGT GGCGGTGATG CAGACGGCGG TTTAGGCTCA AATGTCTCTT TAGGCAACAC
2761 AGTCGGCACC TCAACTATTG TACTGGTTTC GGGCGCCGTT TTTGGTTTGA CCGGTCTGAG
2821 ACGAGTGCGA TTTTTTTCGT TTCTAATAGC TTCCAACAAT TGTTGTCTGT CGTCTAAAGG
2881 TGCAGCGGGT TGAGGTTCCG TCGGCATTGG TGGAGCGGGC GGCAATTCAG ACATCGATGG
2941 TGGTGGTGGT GGTGGAGGCG CTGGAATGTT AGGCACGGGA GAAGGTGGTG GCGGCGGTGC
3001 CGCCGGTATA ATTTGTTCTG GTTTAGTTTG TTCGCGCACG ATTGTGGGCA CCGGCGCAGG
3061 CGCCGCTGGC TGCACAACGG AAGGTCGTCT GCTTCGAGGC AGCGCTTGGG GTGGTGGCAA
3121 TTCAATATTA TAATTGGAAT ACAAATCGTA AAAATCTGCT ATAAGCATTG TAATTTCGCT
3181 ATCGTTTACC GTGCCGATAT TTAACAACCG CTCAATGTAA GCAATTGTAT TGTAAAGAGA
3241 TTGTCTCAAG CTCGGAACGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA
3301 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG
3361 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA
3421 TAGGCTCCGC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC
3481 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT
3541 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG
3601 CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG
3661 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT
3721 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG
3781 ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC
3841 GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA
3901 AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT
3961 GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGTTACCA
4021 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC
4081 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC
4141 TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC
4201 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT
4261 TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT
4321 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC
4381 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG
4441 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT
4501 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC
4561 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG
4621 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT
4681 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC
4741 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC
4801 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA
4861 ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG
4921 TCTCATGTCC GCGCGTTTCC TGCATCTTTT AATCAAATCC CAAGATGTGT ATAAACCACC
4981 AAACTGCCAA AAAATGAAAA CTGTCGACAA GCTCTGTCCG TTTGCTGGCA ACTGCAAGGG
5041 TCTCAATCCT ATTTGTAATT ATTGAATAAT AAAACAATTA TAAATGTCAA ATTTGTTTTT
5101 TATTAACGAT ACAAACCAAA CGCAACAAGA ACATTTGTAG TATTATCTAT AATTGAAAAC
5161 GCGTAGTTAT AATCGCTGAG GTAATATTTA AAATCATTTT CAAATGATTC ACAGTTAATT
5221 TGCGACAATA TAATTTTATT TTCACATAAA CTAGACGCCT TGTCGTCTTC TTCTTCGTAT
5281 TCCTTCTCTT TTTCATTTTT CTCTTCATAA AAATTAACAT AGTTATTATC GTATCCATAT
5341 ATGTATCTAT CGTATAGAGT AAATTTTTTG TTGTCATAAA TATATATGTC TTTTTTAATG
5401 GGGTGTATAG TACCGCTGCG CATAGTTTTT CTGTAATTTA CAACAGTGCT ATTTTCTGGT
5461 AGTTCTTCGG AGTGTGTTGC TTTAATTATT AAATTTATAT AATCAATGAA TTTGGGATCG
5521 TCGGTTTTGT ACAATATGTT GCCGGCATAG TACGCAGCTT CTTCTAGTTC AATTACACCA
5581 TTTTTTAGCA GCACCGGATT AACATAACTT TCCAAAATGT TGTACGAACC GTTAAACAAA
5641 AACAGTTCAC CTCCCTTTTC TATACTATTG TCTGCGAGCA GTTGTTTGTT GTTAAAAATA
5701 ACAGCCATTG TAATGAGACG CACAAACTAA TATCACAAAC TGGAAATGTC TATCAATATA
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