LOCUS       pCANTAB 5E             4591 bp    DNA     circular SYN 31-OCT-2013
DEFINITION  pCANTAB5E.
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biovector.net/
COMMENT     VNTAUTHORNAME|biovector.net|
FEATURES             Location/Qualifiers
     promoter        131..159
                     /vntifkey="30"
                     /label=AmpR_promoter
     CDS             201..1061
                     /gene="Ampicillin"
                     /vntifkey="4"
                     /label=Ampicillin
                     /note="ORF frame 3"
     gene            201..1061
                     /gene="Ampicillin"
                     /vntifkey="60"
     rep_origin      1216..1835
                     /vntifkey="33"
                     /label=pBR322_origin
     promoter        2144..2173
                     /vntifkey="30"
                     /label=lac_promoter
     misc_feature    2187..2209
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     misc_feature    2208..2226
                     /vntifkey="21"
                     /label=M13_reverse_primer
     CDS             2218..3713
                     /vntifkey="4"
                     /label=ORF\frame\1
     misc_feature    complement(3723..3739)
                     /vntifkey="21"
                     /label=M13_forward20_primer
     misc_feature    complement(3732..3754)
                     /vntifkey="21"
                     /label=M13_pUC_fwd_primer
     misc_feature    3720..3875
                     /vntifkey="21"
                     /label=lacZ_a
     rep_origin      complement(4067..4373)
                     /vntifkey="33"
                     /label=f1_origin
     misc_feature    4541..4563
                     /vntifkey="21"
                     /label=pGEX_3_primer
     misc_feature    2329..2342
                     /vntifkey="21"
                     /label=VH
     misc_feature    2437..2441
                     /vntifkey="21"
                     /label=VL
     misc_feature    2376..2398
                     /vntifkey="21"
                     /label=pCantab5-S3
     misc_feature    complement(2376..2398)
                     /vntifkey="21"
                     /label=pCantab5-S4
     primer          2222..2247
                     /vntifkey="27"
                     /label=pCantab5-R1
     primer          2265..2288
                     /vntifkey="27"
                     /label=pCantab5-S1
     misc_feature    complement(2523..2545)
                     /vntifkey="21"
                     /label=pCantab-S6
     misc_feature    complement(2554..2578)
                     /vntifkey="21"
                     /label=pCantab-R2
     misc_feature    2451..2489
                     /vntifkey="21"
                     /label=E\Tag
     misc_feature    2496..2498
                     /vntifkey="21"
                     /label=Amber\Stop\Codon
BASE COUNT     1112 a      1099 c      1172 g      1196 t        12 others
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 
      301 ctgaagatca gttgggtgct cgagtgggtt acatcgaact ggatctcaac agcggtaaga 
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 
     1561 cgtgcataca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 
     1621 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg 
     2221 accatgatta cgccaagctt tggagccttt tttttggaga ttttcaacgt gaaaaaatta 
     2281 ttattcgcaa ttcctttagt tgttcctttc tatgcggccc agccggccnn nnnnggggcc 
     2341 aaggcaccac ggtcaccgtc tcctcaggtg gaggcggttc aggcggaggt ggctctggcg 
     2401 gtggcggatc ggacatcgag ctcactcagt ctccannnnn ngcggccgca ggtgcgccgg 
     2461 tgccgtatcc ggatccgctg gaaccgcgtg ccgcatagac tgttgaaagt tgtttagcaa 
     2521 aacctcatac agaaaattca tttactaacg tctggaaaga cgacaaaact ttagatcgtt 
     2581 acgctaacta tgagggctgt ctgtggaatg ctacaggcgt tgtggtttgt actggtgacg 
     2641 aaactcagtg ttacggtaca tgggttccta ttgggcttgc tatccctgaa aatgagggtg 
     2701 gtggctctga gggtggcggt tctgagggtg gcggttctga gggtggcggt actaaacctc 
     2761 ctgagtacgg tgatacacct attccgggct atacttatat caaccctctc gacggcactt 
     2821 atccgcctgg tactgagcaa aaccccgcta atcctaatcc ttctcttgag gagtctcagc 
     2881 ctcttaatac tttcatgttt cagaataata ggttccgaaa taggcagggt gcattaactg 
     2941 tttatacggg cactgttact caaggcactg accccgttaa aacttattac cagtacactc 
     3001 ctgtatcatc aaaagccatg tatgacgctt actggaacgg taaattcaga gactgcgctt 
     3061 tccattctgg ctttaatgag gatccattcg tttgtgaata tcaaggccaa tcgtctgacc 
     3121 tgcctcaacc tcctgtcaat gctggcggcg gctctggtgg tggttctggt ggcggctctg 
     3181 agggtggcgg ctctgagggt ggcggttctg agggtggcgg ctctgagggt ggcggttccg 
     3241 gtggcggctc cggttccggt gattttgatt atgaaaaaat ggcaaacgct aataaggggg 
     3301 ctatgaccga aaatgccgat gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt 
     3361 ctgtcgctac tgattacggt gctgctatcg atggtttcat tggtgacgtt tccggccttg 
     3421 ctaatggtaa tggtgctact ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg 
     3481 gtgacggtga taattcacct ttaatgaata atttccgtca atatttacct tctttgcctc 
     3541 agtcggttga atgtcgccct tatgtctttg gcgctggtaa accatatgaa ttttctattg 
     3601 attgtgacaa aataaactta ttccgtggtg tctttgcgtt tcttttatat gttgccacct 
     3661 ttatgtatgt attttcgacg tttgctaaca tactgcgtaa taaggagtct taataagaat 
     3721 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat 
     3781 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat 
     3841 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc 
     3901 cttacgcatc tgtgcggtat ttcacaccgc atataaattg taaacgttaa tattttgtta 
     3961 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc 
     4021 aaaatccctt ataaatcaaa agaatagccc gagatagggt tgagtgttgt tccagtttgg 
     4081 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat 
     4141 cagggcgatg gcccactacg tgaaccatca cccaaatcaa gttttttggg gtcgaggtgc 
     4201 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag 
     4261 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg 
     4321 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta 
     4381 cagggcgcgt actatggttg ctttgacggg tgcagtctca gtacaatctg ctctgatgcc 
     4441 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt 
     4501 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag 
     4561 aggttttcac cgtcatcacc gaaacgcgcg a