p3XFLAG-CMV-8載體-哺乳動(dòng)物細(xì)胞表達(dá)質(zhì)粒

General description

p3XFLAG-CMV^?-8 Expression Vector is a 4.8 kb derivative of pCMV5 used to establish expression of N-terminal 3XFLAG^? fusion proteins in mammalian cells. The vector encodes three adjacent FLAG epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) upstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2 antibody. The third FLAG epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein. The preprotrypsin leader sequence precedes the 3XFLAG sequence.

p3XFLAG-CMV-8 Expression Vector is a shuttle vector for E. coli and mammalian cells. Efficiency of replication is optimal when using an SV40 T antigen-expressing host.

p3XFLAG-CMV-7-BAP Control Plasmid is a 6.2 kb derivative of pCMV5 used for transient intracellular expression of N-terminal 3XFLAG bacterial alkaline phosphatase fusion protein in mammalian cells. The vector encodes three adjacent FLAG epitopes (Asp-Tyr-Lys-Xaa-Xaa-Asp) upstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2 antibody. The third FLAG epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein.

Principle

The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG^? fusion constructs.

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