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熒光表達載體：

熒光表達載體共 879 條

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2017-02-22

pHIV-Luc-ZsGreen慢病毒雙熒光表達載體質粒-BioVector NTCC質粒載體菌種細胞蛋白抗體基因保藏中心

pHIV-Luc-ZsGreen Plasmid 39196 -BioVector NTCC質粒載體菌種細胞蛋白抗體基因保藏中心描述： pHIV-Luc-ZsGreen慢病毒雙熒光表達載體質粒，螢火蟲酶熒光和ZsGreen綠色熒光通過IRES雙順反子元件連接雙表達 Vector backbonepHIV-Zsgreen (Search Vector Database) Total vector size (bp)9312 Modifications to backboneThis plasmid was generated by cloning Luc2P (firefly luciferase) into the EcoRI and XbaI sites of pHIV-ZsGreen (Addgene Plasmid 18121). Vector typeMammalian Expression, Lentiviral GROWTH IN BACTERIA Bacterial Resistance(s)Ampicillin Growth Temperature37°C Growth Strain(s)Stbl3 Copy numberHigh Copy GENE/INSERT 1 Gene/Insert nameFirefly Luciferase Luc2P Insert Size (bp)1600 PromoterEF1-alpha CLONING INFORMATION FOR GENE/INSERT 1 Cloning methodRestriction Enzyme 5′ cloning siteEcoRI (not destroyed) 3′ cloning siteXbaI (not destroyed) 5′ sequencing primerEF1a Fwd Primer (Common Sequencing Primers) GENE/INSERT 2 Gene/Insert nameZsGreen Insert Size (bp)700 PromoterEF1-alpha CLONING INFORMATION FOR GENE/INSERT 2 Cloning methodRestriction Enzyme 5′ cloning siteMscI (not destroyed) 3′ cloning siteClaI (not destroyed) 5′ sequencing primern/a 3′ sequencing primerpBluescriptKS Primer pHIV-Luc-ZsGreen慢病毒雙熒光表達載體質粒，螢火蟲酶熒光和ZsGreen綠色熒光通過IRES雙順反子元件連接雙表達.png? >Full plasmid sequence gtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcata gttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagct acaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttc gcgatgtacgggccagatatacgcgttgacattgattattgactagttattaatagtaatcaattacggg gtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctga ccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactt tccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatat gccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgacc ttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggtttt ggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgt caatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattg acgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagcgcgttttgcctgtactgggtct ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaat aaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccct cagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacttgaaagcgaaaggga aaccagaggagctctctcgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcg actggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagt attaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaac atcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttaga tcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaag ctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggccggccgcgctga tcttcagacctggaggaggagatatgagggacaattggagaagtgaattatataaatataaagtagtaaa aattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtg ggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgc tgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattga ggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtg gaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccactg ctgtgccttggaatgctagttggagtaataaatctctggaacagatttggaatcacacgacctggatgga gtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaa gaaaagaatgaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaa attggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgc tgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacctcccaacc ccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagatccattc gattagtgaacggatcggcactgcgtgcgccaattctgcagacaaatggcagtattcatccacaatttta aaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacataca aactaaagaattacaaaaacaaattacaaaaattcaaaattttcgggtttattacagggacagcagagat ccagtttggttagtaccgggcccgctctagccgtgaggctccggtgcccgtcagtgggcagagcgcacat cgcccacagtccccgagaagttggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcgg ggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatata agtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgt gtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgccttgaattacttccacctggct gcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaa ggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtgcgaatctggt ggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgc gacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggttttt ggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgc ggccaccgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgcc gtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccg cttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcac ccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgcc gtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttaggttggggggaggggttttat gcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattct ccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagttt ttttcttccatttcaggtgtcgtgagcggccgctgaactgAATTCATGgaagatgccaaaaacattaaga agggcccagcgccattctacccactcgaagacgggaccgccggcgagcagctgcacaaagccatgaagcg ctacgccctggtgcccggcaccatcgcctttaccgacgcacatatcgaggtggacattacctacgccgag tacttcgagatgagcgttcggctggcagaagctatgaagcgctatgggctgaatacaaaccatcggatcg tggtgtgcagcgagaatagcttgcagttcttcatgcccgtgttgggtgccctgttcatcggtgtggctgt ggccccagctaacgacatctacaacgagcgcgagctgctgaacagcatgggcatcagccagcccaccgtc gtattcgtgagcaagaaagggctgcaaaagatcctcaacgtgcaaaagaagctaccgatcatacaaaaga tcatcatcatggatagcaagaccgactaccagggcttccaaagcatgtacaccttcgtgacttcccattt gccacccggcttcaacgagtacgacttcgtgcccgagagcttcgaccgggacaaaaccatcgccctgatc atgaacagtagtggcagtaccggattgcccaagggcgtagccctaccgcaccgcaccgcttgtgtccgat tcagtcatgcccgcgaccccatcttcggcaaccagatcatccccgacaccgctatcctcagcgtggtgcc atttcaccacggcttcggcatgttcaccacgctgggctacttgatctgcggctttcgggtcgtgctcatg taccgcttcgaggaggagctattcttgcgcagcttgcaagactataagattcaatctgccctgctggtgc ccacactatttagcttcttcgctaagagcactctcatcgacaagtacgacctaagcaacttgcacgagat cgccagcggcggggcgccgctcagcaaggaggtaggtgaggccgtggccaaacgcttccacctaccaggc atccgccagggctacggcctgacagaaacaaccagcgccattctgatcacccccgaaggggacgacaagc ctggcgcagtaggcaaggtggtgcccttcttcgaggctaaggtggtggacttggacaccggtaagacact gggtgtgaaccagcgcggcgagctgtgcgtccgtggccccatgatcatgagcggctacgttaacaacccc gaggctacaaacgctctcatcgacaaggacggctggctgcacagcggcgacatcgcctactgggacgagg acgagcacttcttcatcgtggaccggctgaagagcctgatcaaatacaagggctaccaggtagccccagc cgaactggagagcatcctgctgcaacaccccaacatcttcgacgccggggtcgccggcctgcccgacgac gatgccggcgagctgcccgccgcagtcgtcgtgctggaacacggtaaaaccatgaccgagaaggagatcg tggactatgtggccagccaggttacaaccgccaagaagctgcgcggtggtgttgtgttcgtggacgaggt gcctaaaggactgaccggcaagttggacgcccgcaagatccgcgagattctcattaaggccaagaagggc ggcaagatcgccgtgtaataattctagagtacccgggctaggatccgcccctctccctccccccccccta acgttactggccgaagccgcttggaataaggccggtgtgcgtttgtctatatgttattttccaccatatt gccgtcttttggcaatgtgagggcccggaaacctggccctgtcttcttgacgagcattcctaggggtctt tcccctctcgccaaaggaatgcaaggtctgttgaatgtcgtgaaggaagcagttcctctggaagcttctt gaagacaaacaacgtctgtagcgaccctttgcaggcagcggaaccccccacctggcgacaggtgcctctg cggccaaaagccacgtgtataagatacacctgcaaaggcggcacaaccccagtgccacgttgtgagttgg atagttgtggaaagagtcaaatggctctcctcaagcgtattcaacaaggggctgaaggatgcccagaagg taccccattgtatgggatctgatctggggcctcggtAcacatgctttacatgtgtttagtcgaggttaaa aaaacgtctaggccccccgaaccacggggacgtggttttcctttgaaaaacacgatgataatatggccac aaccatggcccagtccaagcacggcctgaccaaggagatgaccatgaagtaccgcatggagggctgcgtg gacggccacaagttcgtgatcaccggcgagggcatcggctaccccttcaagggcaagcaggccatcaacc tgtgcgtggtggagggcggccccttgcccttcgccgaggacatcttgtccgccgccttcatgtacggcaa ccgcgtgttcaccgagtacccccaggacatcgtcgactacttcaagaactcctgccccgccggctacacc tgggaccgctccttcctgttcgaggacggcgccgtgtgcatctgcaacgccgacatcaccgtgagcgtgg aggagaactgcatgtaccacgagtccaagttctacggcgtgaacttccccgccgacggccccgtgatgaa gaagatgaccgacaactgggagccctcctgcgagaagatcatccccgtgcccaagcagggcatcttgaag ggcgacgtgagcatgtacctgctgctgaaggacggtggccgcttgcgctgccagttcgacaccgtgtaca aggccaagtccgtgccccgcaagatgcccgactggcacttcatccagcacaagctgacccgcgaggaccg cagcgacgccaagaaccagaagtggcacctgaccgagcacgccatcgcctccggctccgccttgccctaa ggatcgcatcgataccgtcgacctcgatcgagacctagaaaaacatggagcaatcacaagtagcaataca gcagctaccaatgctgattgtgcctggctagaagcacaagaggaggaggaggtgggttttccagtcacac ctcaggtacctttaagaccaatgacttacaaggcagctgtagatcttagccactttttaaaagaaaaggg gggactggaagggctaattcactcccaacgaagacaagatatccttgatctgtggatctaccacacacaa ggctacttccctgattggcagaactacacaccagggccagggatcagatatccactgacctttggatggt gctacaagctagtaccagttgagcaagagaaggtagaagaagccaatgaaggagagaacacccgcttgtt acaccctgtgagcctgcatgggatggatgacccggagagagaagtattagagtggaggtttgacagccgc ctagcatttcatcacatggcccgagagctgcatccggactgtactgggtctctctggttagaccagatct gagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgct tcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtg tggaaaatctctagcagcatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgc tggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcg aaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccg accctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcac gctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttca gcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgcca ctggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagt ggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttacctt cggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgc aagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacg ctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagat ccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttac caatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactcc ccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgaga cccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggt cctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccag ttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggc ttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggtt agctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcag cactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaa gtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcg ccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatct taccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttacttt caccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacgg aaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatga gcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagt gccacctgac

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